Evidence map›Paper›PMID 40009329›Full record

ArticleProbiotics and antimicrobial proteins2025

Genomic Scale Analysis Foresees Enteroprotective and Butyrogenic Properties in Brazilian Isolates of Lactiplantibacillus plantarum.

Thayra Gomes Dos Santos, Karina Araujo Gomes Dos Santos, Eddy José Francisco de Oliveira, Bertram Brenig, Elinalva Maciel Paulo, Pedro Henrique Marques, Valbert Nascimento Cardoso, Flávia Figueira Aburjaile, Siomar Castro Soares, Wanderson Marques da Silva and 2 more

Abstract read
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In one paragraph

Article in Probiotics and antimicrobial proteins, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Gut-kidney axis: Dysbiosis and renal disease.World journal of nephrology · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Thayra Gomes Dos SantosDepartment of Biochemistry and Biophysics, Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil.
Karina Araujo Gomes Dos SantosDepartment of Biochemistry and Biophysics, Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil.
Eddy José Francisco de OliveiraLaboratory of Applied Microbiology and Public Health, State University of Feira de Santana, Feira de Santana, Brazil.
Bertram BrenigInstitute of Veterinary Medicine, University of Göttingen, Göttingen, Germany.
Elinalva Maciel PauloLaboratory of Applied Microbiology and Public Health, State University of Feira de Santana, Feira de Santana, Brazil.
Pedro Henrique MarquesDepartment of Genetics, Ecology and Evolution, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Valbert Nascimento CardosoLaboratory of Radioisotopes, Department of Clinical Analysis and Toxicology, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Flávia Figueira AburjailePreventive Veterinary Medicine Departament, Veterinary School, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Siomar Castro SoaresDepartment of Microbiology, Immunology and Parasitology, Federal University of Triangulo Mineiro, Uberaba, Brazil.
Wanderson Marques da SilvaInstitute of Agrobiotechnology and Molecular Biology (IABIMO), INTA-CONICET, Hurlingham, Argentina.
Vasco AzevedoDepartment of Genetics, Ecology and Evolution, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Rodrigo Dias de Oliveira CarvalhoDepartment of Biochemistry and Biophysics, Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil. rodrigodoc2@gmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Probiogenomics offers a rapid and comprehensive approach to characterizing the beneficial properties of microorganisms, allowing the identification and selection of specific strains for improving human health. The species Lactiplantibacillus plantarum is highly ubiquitous and presents great genetic diversity making it difficult to conduct massive screening of probiotic strains when using conventional methods. Hence, this work aimed to taxonomically and functionally characterize the complete genomes of six novel L. plantarum strains isolated in Brazil through high-standard NGS. The taxonomy of strains E6, E10, E14, E18, Lpl2, and Lpl4 was investigated through average nucleotide identity analysis using the genome of L. plantarum SK151 from the GENBANK database as a reference. Moreover, multilocus phylogenetic analyses were performed to investigate their genetic relatedness to probiotic L. plantarum strains using a total of 225 complete genomes from the same database. To investigate specific health-promoting and safety properties, the isolates from Brazil were analyzed using several bioinformatic tools to predict antimicrobials and anti-inflammatory biomolecules and potential risk factors including drug resistance, virulence genes, and genetic mobility elements. The results confirmed that all six isolates belong to the species L. plantarum and the phylogenetic analysis suggests high proximity to known probiotics and strains that have demonstrated protective effects against inflammation and pathogens. Several bacteriocin genes were identified in the Brazilian isolates, mostly plantaricins, suggesting antimicrobial properties, especially in the strain E14 that presented 9 bacteriocins genes. Furthermore, the study revealed the potential capacity of these strains to produce anti-inflammatory compounds including metabolites such as butyrate through the fermentation of fructan sugars, and IL-10 stimulation activity mediated by adhesin proteins from the bacterial cell wall. The lpl4 strain glutamate decarboxylase (GADB) gene showed a high degree of genetic and structural conservation prediction and increased stabilization of the predicted protein compared to a functional homologous GADB from L. plantarum Taj-Apis362, suggesting lpl4 potential for the modulation of the gut-brain axis functions by the production of GABA. Regarding the presence of virulence and resistance genes, only one resistance island was observed in strain E6 genome. In conclusion, it is possible to suggest that most of the evaluated strains are safe and we highlight the strains Lpl4 and E14 for presenting outstanding enteroprotective properties such as the potential production of GABA and several bacteriocins.

Indexed as

Genome, BacterialLactiplantibacillus plantarumProbioticsBrazilHumansPhylogenyAntimicrobial peptidesBioprospectingGABAGut-brain axisLactic acid bacteriaOmics

Identifiers

PMID40009329

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.