Evidence map›Paper›PMID 40008431›Full record

ArticleCNS neuroscience & therapeutics2025

Free and Easy Wanderer Ameliorates Intestinal Bloating-Dependent Avoidance Behavior of Caenorhabditis elegans Through Gut-Germline-Neural Signaling.

Siyi Lin, Huangjie Fu, Hanxiao Wang, Yingying Xu, Yu Zhao, Shiyu Du, Jiale Wei, Ping Qiu, Senlin Shi, Changyu Li and 2 more

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Article in CNS neuroscience & therapeutics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

12 authors.

Siyi LinSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, China.
Huangjie FuSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, China.
Hanxiao WangSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, China.
Yingying XuSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, China.
Yu ZhaoSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, China.
Shiyu DuSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, China.
Jiale WeiSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, China.
Ping QiuSchool of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, China.
Senlin ShiSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, China.
Changyu LiSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, China.
Thomas EfferthDepartment of Pharmaceutical Biology, Institute of Pharmaceutical and Biomedical Sciences, Johannes Gutenberg University, Mainz, Germany.
Chunlan HongSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, China.ORCID 0000-0002-8671-7138

Funding

National Natural Science Foundation of China 82104423
6 · The paper itself

Abstract

purposeThe aim of this study was to investigate the protective effect of Free and Easy Wanderer (FAEW) on the avoidance behavior induced by feeding Heat-Killed Escherichia coli, and to elucidate the underlying mechanisms.

methodsInitially, the effects of FAEW on avoidance behavior, survival, neuroendocrine signaling gene expression, and intestinal bloating were examined. The impact of FAEW on gut-germline-neural signaling was assessed by monitoring H4K8ac expression and the avoidance behavior of par-5 RNAi animals and glp-1(e2141) mutants. RNA-sequencing was conducted to analyze potential signaling pathways. Finally, avoidance behavior was examined using daf-16(mu86) mutants and the rescued animals.

resultsFAEW delayed avoidance behavior. FAEW significantly downregulated gene expression in the neuroendocrine signaling pathway and alleviated intestinal bloating of C. elegans. The levels of H4K8ac and par-5 in the germline decreased significantly with FAEW's treatment, and FAEW failed to affect the avoidance behavior of par-5 RNAi animals and glp-1(e2141) mutants. FAEW's effect on avoidance behavior diminished in daf-16(mu86) mutants but was restored in daf-16 rescued animals. FAEW has been observed to restore daf-16 levels.

conclusionFAEW protects against avoidance behavior of C. elegans through downregulating H4K8ac protein expression and activating DAF-16. This study provides crucial experimental evidence supporting FAEW as a promising candidate for protecting against avoidance behavior associated with PTSD.

Indexed as

Avoidance LearningAnimalsAnimals, Genetically ModifiedCaenorhabditis elegansCaenorhabditis elegans ProteinsSignal TransductionCaenorhabditis elegans Proteinsfree and easy wanderergut‐germline‐neural signalingH4K8acintestinal bloating

Identifiers

PMID40008431
PMCPMC11862825

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