Evidence map›Paper›PMID 40007034›Full record

ArticleViruses2025

Deciphering Viral Replication Dynamics in Feline Infectious Peritonitis: A Quantitative Approach.

Carole J Burgener, Emi N Barker, Teodoru Soare, Diana-Gabriela Soare, Andrea M Spiri, Alexandra J Malbon, Marina L Meli, Anja Kipar

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Carole J BurgenerInstitute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich (IVPZ), Winterthurerstrasse 268, 8057 Zürich, Switzerland.
Emi N BarkerLangford Vets, University of Bristol, Langford BS40 5DU, UK.ORCID 0000-0001-6293-6662
Teodoru SoareDepartment of Pathology, Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine of Bucharest, Splaiul Independentei Street, No. 105, Sector 5, 050097 Bucharest, Romania.ORCID 0000-0001-8034-8549
Diana-Gabriela SoareHistovet, Str. Sirenelor 81-83, Sector 5, 50855 Bucharest, Romania.
Andrea M SpiriCenter for Clinical Studies, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 260, 8057 Zurich, Switzerland.ORCID 0000-0001-9009-8256
Alexandra J MalbonThe Royal (Dick) School of Veterinary Studies, Easter Bush Campus, University of Edinburgh, Edinburgh EH8 9YL, UK.ORCID 0000-0002-5144-3333
Marina L MeliCenter for Clinical Studies, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 260, 8057 Zurich, Switzerland.ORCID 0000-0002-3609-2416
Anja KiparInstitute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich (IVPZ), Winterthurerstrasse 268, 8057 Zürich, Switzerland.ORCID 0000-0001-7289-3459

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Feline infectious peritonitis (FIP) is a complex immune-mediated disease caused by feline coronavirus (FCoV). Despite advancements in understanding its pathogenesis, challenges persist in elucidating viral factors related to virion composition and replication. This study examined FCoV-infected cats with and without FIP for potential associations with or variation in expression ratios for different viral genes. We analyzed tissue samples with FIP lesions from 46 cats with FIP and mesenteric lymph nodes from 10 FCoV-infected cats (7b RT-qPCR positive) without FIP with three RT-qPCR assays, targeting (sub)-genomic RNAs of the RNA-dependent RNA polymerase (RdRp) and envelope (E) genes. In cats with FIP, the RdRp mRNA assay yielded the highest copy numbers, followed by the combined RdRp gRNA and mRNA assay; the E mRNA assay yielded the lowest copy numbers. In cats without FIP, significantly fewer viral RNA copies were detected regardless of the assay. Viral gene expression was not detected in six and was observed only at low levels in one or more assays in four samples. The observed correlation between assays and the intragroup correlation assay indicate consistent transcription of both the structural E protein and RdRp genes within FIP lesions in cats with FIP but not in cats with systemic FCoV infection alone.

Indexed as

Coronavirus, FelineFeline Infectious PeritonitisVirus ReplicationAnimalsCatsFemaleMaleRNA-Dependent RNA PolymeraseRNA, ViralViral LoadRNA-Dependent RNA PolymeraseRNA, Viralenvelope proteinFCoVFIPRNA-dependent RNA polymeraseRT-qPCRviral genomic and messenger RNA

Identifiers

PMID40007034
PMCPMC11860597

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.