Evidence map›Paper›PMID 40006656›Full record

ArticleVaccines2025

Development of a Recombinase-Mediated Cassette Exchange System for Gene Knockout and Expression of Non-Native Gene Sequences in

Benjamin Cull, Nicole Y Burkhardt, Benedict S Khoo, Jonathan D Oliver, Xin-Ru Wang, Lisa D Price, Kamil Khanipov, Rong Fang, Ulrike G Munderloh

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In one paragraph

Article in Vaccines, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Benjamin CullDepartment of Entomology, College of Food, Agricultural and Natural Resource Sciences, University of Minnesota, St. Paul, MN 55108, USA.ORCID 0000-0001-6814-5669
Nicole Y BurkhardtDepartment of Entomology, College of Food, Agricultural and Natural Resource Sciences, University of Minnesota, St. Paul, MN 55108, USA.
Benedict S KhooDivision of Environmental Health Sciences, School of Public Health, University of Minnesota, Minneapolis, MN 55455, USA.
Jonathan D OliverDivision of Environmental Health Sciences, School of Public Health, University of Minnesota, Minneapolis, MN 55455, USA.ORCID 0000-0002-8738-112X
Xin-Ru WangDepartment of Entomology, College of Food, Agricultural and Natural Resource Sciences, University of Minnesota, St. Paul, MN 55108, USA.
Lisa D PriceDepartment of Entomology, College of Food, Agricultural and Natural Resource Sciences, University of Minnesota, St. Paul, MN 55108, USA.ORCID 0009-0002-0833-4032
Kamil KhanipovDepartment of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston, TX 77555, USA.ORCID 0000-0002-3881-737X
Rong FangDepartment of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USA.
Ulrike G MunderlohDepartment of Entomology, College of Food, Agricultural and Natural Resource Sciences, University of Minnesota, St. Paul, MN 55108, USA.

Funding

Rational development of a vaccine against tick-borne rickettsiosesR01AI163857 · NIAID · UNIVERSITY OF TEXAS MED BR GALVESTON · PI FANG, RONG MEGAN · 2022 to 2025
$3.2M
Identification of diagnostic biomarkers for flea-borne typhusR21AI183177 · NIAID · UNIVERSITY OF TEXAS MED BR GALVESTON · PI Rong Megan Fang · 2025 to 2026
$433k
NIAID NIH HHS R01 AI163857NIAID NIH HHS R21 AI183177United States Department of Defense W81XWH-18-1-0319
6 · The paper itself

Abstract

BACKGROUND/

objectivesIncidence of vector-borne diseases, including rickettsioses and anaplasmosis, has been increasing in many parts of the world. The obligate intracellular nature of rickettsial pathogens has hindered the development of robust genetic tools for the study of gene function and the identification of therapeutic targets. Transposon mutagenesis has contributed to recent progress in the identification of virulence factors in this important group of pathogens.

methodsCombining the efficiency of the himar1 transposon method with a recombinase-mediated system, we aimed to develop a genetic tool enabling the exchange of the transposon with a cassette encoding non-native sequences.

resultsThis approach was used in

conclusionsThe use of this system provides a well-established and relatively efficient way of inserting non-native sequences into the rickettsial genome, with applications for the study of gene function and vaccine development.

Indexed as

Anaplasmagenetic toolslive-attenuated vaccineRickettsiatransposon mutagenesis

Identifiers

PMID40006656
PMCPMC11861799

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.