ArticleVeterinary sciences2025
Application of
Article in Veterinary sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Opposing fates: a bipolar cellular model for FMDV replication shaped byFrontiers in cellular and infection microbiology · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
FMD poses a significant threat to animal husbandry and public health security. This study aims to investigate an innovative method for producing FMD vaccines. Wild-type BHK-21 cells were subjected to heavy ion irradiation. Following the optimization of irradiation parameters, the mutant cell line BHK-7 was selected using the limited dilution method. The concentration of FMDV 146S in the BHK-7 cells was markedly elevated, significantly enhancing FMDV replication. The suspension culture and domestication experiments demonstrated that BHK-7 exhibited characteristics like those of the control BHK-21 cells, thereby improving production efficiency and reducing costs. The metabolic analysis of the BHK-7 suspension cultures indicated that glutamine (GLN) may play a crucial role in FMDV replication, with the addition of an appropriate amount of GLN enhancing viral replication levels. Ten successive generations of BHK-7 cells showed stability in FMDV replication post-domestication, indicating good genetic stability. In this study, we obtained a mutant somatic cell line, BHK-7, which promotes FMDV replication through heavy ion irradiation technology. Through suspension culture domestication and metabolic analysis, this study provides a novel approach and concept for FMD vaccine production, as well as a reference for the development of other vaccine cell lines.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.