Evidence map›Paper›PMID 40005847›Full record

ArticleVeterinary sciences2025

Melatonin Modulates ZAP70 and CD40 Transcripts via Histone Modifications in Canine Ileum Epithelial Cells.

Jian Hong, Saber Y Adam, Shiqi Wang, Hao Huang, In Ho Kim, Abdelkareem A Ahmed, Hao-Yu Liu, Demin Cai

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Jian HongSchool of Marine and Biological Engineering, Yancheng Teachers University, Yancheng 224002, China.
Saber Y AdamCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Shiqi WangCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Hao HuangCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
In Ho KimDepartment of Animal Resource and Science, Dankook University, Cheonan 100000, Republic of Korea.ORCID 0000-0001-6652-2504
Abdelkareem A AhmedDepartment of Veterinary Sciences, Botswana University of Agriculture and Natural Resources, Gaborone P.O. Box 100, Botswana.ORCID 0000-0003-4015-2845
Hao-Yu LiuCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Demin CaiCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.ORCID 0000-0003-0500-5292

Funding

the construction of doctoral programs in Traditional Chinese Medicine (Tibetan Medicine), and the cultivation of doctoral programs in Traditional Chinese Pharmacy (Tibetan Pharmacy) (BSDJS-BH-23-01) (BSDJS-BH-23-02)the Natural Science Foundation of Jiangsu Province BK20220582, BK20210812the Open Project Program of the International Joint Research Laboratory in Universities of Jiangsu Province of China for Domestic Animal Germplasm Resources and Genetic Improvement, the Priority Academic Program Development of Jiangsu Higher Education Ins PAPD
6 · The paper itself

Abstract

Melatonin (MLT), produced by the pineal gland and other tissues, is known for its anti-inflammatory effects, particularly in regulating inflammatory markers and cytokines in intestinal cells. Our study aimed to investigate how MLT influences the expression of inflammatory genes through histone modification in canine ileum epithelial cells (cIECs). In our experiment, cIECs were cultured and divided into a control group (CON) and an MLT-treatment group. MLT did not significantly affect cell growth or death in cIECs compared to the CON. However, MLT treatment led to an upregulation of CD40, ZAP70, and IL7R and a downregulation of LCK, RPL37, TNFRSF13B, CD4, CD40LG, BLNK, and CIITA at the mRNA expression level. Moreover, MLT significantly altered the NF-kappa B signaling pathway by upregulating genes, such as CD40, ZAP70, TICAM1, VCAMI, GADD45B, IRAK1, TRADD, RELA, RIPK1, and RELB, and downregulating PRKCB, LY96, CD40LG, ILIB, BLNK, and TNFRSF11A. Using ChIP-qPCR, we discovered that MLT treatment enhanced histone acetylation marks H3K9ac, H3K18ac, H3K27ac, and methylation marks H3K4me1 and H3K4me3 at the ZAP70 and CD40 gene loci (

Indexed as

caninehistone modificationsileummelatonintranscriptome

Identifiers

PMID40005847
PMCPMC11860356

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.