Evidence map›Paper›PMID 40005596›Full record

ArticleMicroorganisms2025

The Impact of Viral Concentration Method on Quantification and Long Amplicon Nanopore Sequencing of SARS-CoV-2 and Noroviruses in Wastewater.

George Scott, Nicholas P Evens, Jonathan Porter, David I Walker

Abstract read
In one paragraph

Article in Microorganisms, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

George ScottCentre for Environment, Fisheries and Aquaculture Science, The Nothe, Barrack Road, Weymouth DT4 8UB, UK.ORCID 0000-0002-4468-4366
Nicholas P EvensEnvironment Agency, National Monitoring, Starcross, Exeter EX6 8FD, UK.
Jonathan PorterEnvironment Agency, National Monitoring, Starcross, Exeter EX6 8FD, UK.ORCID 0000-0003-3204-5257
David I WalkerCentre for Environment, Fisheries and Aquaculture Science, The Nothe, Barrack Road, Weymouth DT4 8UB, UK.

Funding

Department for Environment, Food and Rural Affairs NAHis Majesty's Treasury Shared Outcome Fund PATH-SAFE programme NAUK Health Security Agency NAUnited Kingdom Government (Department of Health and Social Care) NA
6 · The paper itself

Abstract

Wastewater-based surveillance has gained attention in the four years following the start of the COVID-19 pandemic. Accurate pathogen detection, quantification and characterisation rely on the selection of appropriate methodologies. Here, we explore the impact of viral concentration method on RT-qPCR inhibition and quantification of norovirus genogroups I and II (GI and GII), crAssphage, phi6 and SARS-CoV-2. Additionally, their impact on long amplicon sequencing for typing noroviruses and whole-genome sequencing (WGS) SARS-CoV-2 was explored. RT-qPCR inhibition for each viral concentration method was significantly different apart from the two ultrafiltration methods, InnovaPrep

Indexed as

crAssphagelong ampliconnanopore sequencingnorovirusqPCRSARS-CoV-2viral concentrationwastewaterwastewater surveillancewhole-genome sequencing

Identifiers

PMID40005596
PMCPMC11857638

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.