Evidence map›Paper›PMID 40005539›Full record

ArticlePathogens (Basel, Switzerland)2025

Advanced rDNA-Based Detection of Wheat Pathogens in Grain Samples Using Next-Generation Sequencing (NGS).

Katarzyna Pieczul, Ilona Świerczyńska, Andrzej Wójtowicz

Abstract read
In one paragraph

Article in Pathogens (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Katarzyna PieczulInstitute of Plant Protection-National Research Institute, 60-318 Poznan, Poland.ORCID 0000-0001-6856-3211
Ilona ŚwierczyńskaInstitute of Plant Protection-National Research Institute, 60-318 Poznan, Poland.
Andrzej WójtowiczInstitute of Plant Protection-National Research Institute, 60-318 Poznan, Poland.ORCID 0000-0003-1455-1527

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

High-throughput sequencing (HTS) has revolutionized phytopathology by overcoming many limitations of traditional diagnostic methods, as it permits precise pathogen monitoring, identification, and control, with ribosomal DNA (rDNA) regions serving as reliable markers for fungal classification. In this study, next-generation sequencing (NGS) was used, targeting the ITS1 and ITS2 regions to explore fungal diversity and pathogen presence in winter wheat grain samples and identifying 183 OTU sequences across 115 taxa. The ITS1 analysis yielded 249,743 reads, with

Indexed as

DNA, RibosomalEdible GrainFungiHigh-Throughput Nucleotide SequencingPlant DiseasesTriticumDNA, FungalDNA, Ribosomal SpacerFusariumPhylogenyDNA, FungalDNA, RibosomalDNA, Ribosomal SpacerfungiITS1 and ITS2next-generation sequencing (NGS)pathogen identificationwheat

Identifiers

PMID40005539
PMCPMC11858152

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.