Evidence map›Paper›PMID 40004973›Full record

ArticleNutrients2025

Anticancer and Antioxidant Effects of Bioactive Peptides from Black Soldier Fly Larvae (

Kwanchanok Praseatsook, Arpamas Vachiraarunwong, Sirinya Taya, Phatthawin Setthaya, Kenji Sato, Hideki Wanibuchi, Rawiwan Wongpoomchai, Pornngarm Dejkriengkraikul, Min Gi, Supachai Yodkeree

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PubMed Full text at PMC Publisher
In one paragraph

Article in Nutrients, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Kwanchanok PraseatsookDepartment of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.ORCID 0000-0001-9274-2869
Arpamas VachiraarunwongDepartment of Environmental Risk Assessment, Osaka Metropolitan University Graduate School of Medicine, Osaka 545-8585, Japan.ORCID 0000-0002-0463-2289
Sirinya TayaFunctional Food Research Unit, Multidisciplinary Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand.ORCID 0000-0002-5681-4279
Phatthawin SetthayaScience and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand.
Kenji SatoDivision of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
Hideki WanibuchiDepartment of Environmental Risk Assessment, Osaka Metropolitan University Graduate School of Medicine, Osaka 545-8585, Japan.
Rawiwan WongpoomchaiDepartment of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.ORCID 0000-0001-6999-8436
Pornngarm DejkriengkraikulDepartment of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.ORCID 0000-0001-8732-8911
Min GiDepartment of Environmental Risk Assessment, Osaka Metropolitan University Graduate School of Medicine, Osaka 545-8585, Japan.ORCID 0000-0003-1642-8127
Supachai YodkereeDepartment of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.ORCID 0000-0001-9023-2283

Funding

This research was funded by the National Research Council of Thailand (NRCT) and Exofood Thailand Company Limited N41A650432
6 · The paper itself

Abstract

backgroundProtein hydrolysates from insects are recognized for their biological activities. Black soldier fly larvae (BSFL) have drawn attention due to their antioxidant protein hydrolysates. However, research on bioactive peptides derived from these hydrolysates, particularly their cancer chemopreventive potential, remains limited. This study aims to evaluate the antioxidant, anti-inflammatory, antimutagenic, and anticancer activities of BSFL-derived bioactive peptides and explore the molecular mechanisms.

methodsAlkali-soluble BSFL protein (ASBP) was extracted and hydrolyzed using Alcalase and bromelain under optimized conditions. Antioxidant activity was assessed via FRAP, ABTS, and DPPH assays. The hydrolysate with the highest antioxidant activity was fractionated into molecular weight (MW) groups (>30, 10, and <3 kDa). The bioactivity of fractionated peptides was evaluated through antioxidant, anti-inflammatory (nitric oxide production in RAW 264.7 cells), antimutagenic (Ames test), and anticancer (CCK-8 assay on HCT 116, COLO205, Cw-2, and Caco-2 cells) assays. Mechanistic insights were obtained via microarray and Western blot analyses. Peptides were identified by LC-MS/MS.

resultsThe ASBP-Alcalase hydrolysate (ASBP-AH) showed optimal antioxidant activity at 3% (

conclusionsBSFL-derived bioactive peptides exhibit potential as multifunctional agents for cancer chemoprevention.

Indexed as

Antineoplastic AgentsAntioxidantsDipteraInsect ProteinsPeptidesAnimalsAnti-Inflammatory AgentsAntimutagenic AgentsCaco-2 CellsCell Line, TumorHumansLarvaMiceNitric OxideProtein HydrolysatesRAW 264.7 CellsAnti-Inflammatory AgentsAntimutagenic AgentsAntineoplastic AgentsAntioxidantsInsect ProteinsNitric OxidePeptidesProtein Hydrolysatesanticancer activityanti-inflammatory activityantimutagenicityantioxidant activityblack soldier fly larvaecolon cancerinsect protein hydrolysates

Identifiers

PMID40004973
PMCPMC11858422

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.