Evidence map›Paper›PMID 40004461›Full record

ArticleGenes2025

Transcriptomic Profiling Highlights Metabolic and Biosynthetic Pathways Involved in In Vitro Flowering in

Shuisheng Yu, Julian Liu, Chenchen Cai, Yi Zhang, Shuangbin Fu, Yanping Yang, Zhuang Zhou, Zhen Ying

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Genes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Shuisheng YuZhejiang Jiulongshan National Nature Reserve Management Center, Lishui 323312, China.
Julian LiuZhejiang Jiulongshan National Nature Reserve Management Center, Lishui 323312, China.
Chenchen CaiZhejiang Jiulongshan National Nature Reserve Management Center, Lishui 323312, China.
Yi ZhangZhejiang Jiulongshan National Nature Reserve Management Center, Lishui 323312, China.
Shuangbin FuZhejiang Institute of Subtropical Crops, Wenzhou 325005, China.ORCID 0000-0002-0822-4848
Yanping YangZhejiang Institute of Subtropical Crops, Wenzhou 325005, China.
Zhuang ZhouZhejiang Institute of Subtropical Crops, Wenzhou 325005, China.ORCID 0000-0002-3894-2576
Zhen YingZhejiang Institute of Subtropical Crops, Wenzhou 325005, China.

Funding

Project of Zhejiang Academy of Agricultural Sciences (Chinese Medicinal Crops-breeding and cultivation of Anoectochilus roxburghii (Wall.) Lindl.) 2024LY27FX05Technology Service Team Project of Ouhai science and technology innovation center of Zhejiang Academy of Agricultural Sciences OHKC2024001The Major Science and Technology Projects of Breeding New Varieties of Agriculture in Zhejiang Province 2021C02074Wenzhou Agricultural New Variety Breeding Cooperation Group ZX2024005-5Wenzhou Major Scientific and Technological Research Project ZN2022008
6 · The paper itself

Abstract

backgroundTissue culture is one of the most important methods for propagating orchids. Notably, many orchid seedlings exhibit autonomous flowering during the cultivation process. To explore the underlying mechanism,

methodsBud samples at the early, middle, and fully open stages were collected for transcriptome sequencing, followed by differential expression, trend, enrichment and protein-protein interaction (PPI) network analyses.

resultsDifferential gene expression analysis identified 2364, 4137, and 6522 differentially expressed genes (DEGs) in the early vs. middle, middle vs. open, and early vs. open comparisons, respectively. These DEGs were significantly enriched in various metabolic and biosynthetic pathways, particularly in ko01100 (metabolic pathways). PPI network analysis further identified hub genes, including

conclusionOur findings provide novel insights into the molecular mechanisms driving in vitro flowering in

Indexed as

Biosynthetic PathwaysFlowersMetabolic Networks and PathwaysOrchidaceaePlant ProteinsTranscriptomeGene Expression ProfilingGene Expression Regulation, PlantProtein Interaction MapsPlant Proteinsbiosynthetic pathwaysDNA replicationflowermetabolic pathwaysRNA-seq

Identifiers

PMID40004461
PMCPMC11855183

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.