Evidence map›Paper›PMID 40001396›Full record

ArticleAntibiotics (Basel, Switzerland)2025

Dynamic Spread of Antibiotic Resistance Determinants by Conjugation to a Human-Derived Gut Microbiota in a Transplanted Mouse Model.

Azam A Sher, Charles E Whitehead-Tillery, Ashley M Peer, Julia A Bell, Daniel B Vocelle, Joshua T Dippel, Lixin Zhang, Linda S Mansfield

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Antibiotics (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Azam A SherComparative Enteric Diseases Laboratory, Departments of Large Animal Clinical Sciences and Microbiology, Genetics and Immunology, East Lansing, MI 48824, USA.ORCID 0000-0002-2607-1291
Charles E Whitehead-TilleryComparative Enteric Diseases Laboratory, Departments of Large Animal Clinical Sciences and Microbiology, Genetics and Immunology, East Lansing, MI 48824, USA.
Ashley M PeerComparative Enteric Diseases Laboratory, Departments of Large Animal Clinical Sciences and Microbiology, Genetics and Immunology, East Lansing, MI 48824, USA.ORCID 0009-0000-3661-2496
Julia A BellComparative Enteric Diseases Laboratory, Departments of Large Animal Clinical Sciences and Microbiology, Genetics and Immunology, East Lansing, MI 48824, USA.
Daniel B VocelleDepartment of Pharmacology and Toxicology, Michigan State University, East Lansing, MI 48824, USA.ORCID 0000-0003-0252-2673
Joshua T DippelComparative Enteric Diseases Laboratory, Departments of Large Animal Clinical Sciences and Microbiology, Genetics and Immunology, East Lansing, MI 48824, USA.
Lixin ZhangDepartments of Epidemiology and Biostatistics and Microbiology, Genetics and Immunology, Michigan State University, East Lansing, MI 48824, USA.
Linda S MansfieldComparative Enteric Diseases Laboratory, Departments of Large Animal Clinical Sciences and Microbiology, Genetics and Immunology, East Lansing, MI 48824, USA.

Funding

Does microbiome composition moderate GI and CNS function in a VPA-induced mouse model of autism?R21HD112101 · NICHD · HENRY FORD HEALTH + MICHIGAN STATE UNIVERSITY HEALTH SCIENCES · PI KNICKMEYER, REBECCA, MANSFIELD, LINDA S. · 2023 to 2023
$430k
Defining the role of the early infant microbiome in mediating allergic outcomes associated with asthma in a mouse modelR21AI121748 · NIAID · MICHIGAN STATE UNIVERSITY · PI EWART, SUSAN L, MANSFIELD, LINDA S. · 2016 to 2017
$426k
Albert C. and Lois E. Dehn Chair Endowment RN031097-DEHNCollege of Veterinary Medicine Stipend to Azam A. SherNational Institutes of Health, Enterics Research Investigational Network, Cooperative Research Center U19AI090872NIAID NIH HHS R21 AI121748NICHD NIH HHS R21 HD112101United States Department of Agriculture NC1202University Distinguished Professor Endowment, Michigan State University GS100019
6 · The paper itself

Abstract

backgroundAntibiotic-resistant (AR) bacteria pose an increasing threat to public health, but the dynamics of antibiotic resistance gene (ARG) spread in complex microbial communities are poorly understood. Conjugation is a predominant direct cell-to-cell mechanism for the horizontal gene transfer (HGT) of ARGs. We hypothesized that commensal

methodsTwo donor

results

conclusionsResults show this ARG-bearing conjugative RP4 plasmid spread to diverse human gut bacterial taxa within a live animal where they persisted. These fluorescent marker strategies and human-derived microbiota transplanted mice provided a tractable model for investigating the dynamic spread of ARGs within gut microbiota and could be applied rigorously to varied microbiotas to understand conditions facilitating their spread.

Indexed as

antibiotic resistancecommensal bacteriaenteric pathogensgut microbesmouseRP4 conjugative plasmid

Identifiers

PMID40001396
PMCPMC11851821

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.