Evidence map›Paper›PMID 40001133›Full record

ArticleCell communication and signaling : CCS2025

LRSAM1 mediated the degradation of intracellular Vibrio through the ubiquitination-autophagy-lysosome pathway in oyster.

Wenwen Yang, Jiejie Sun, Qiuyan Guo, Wei Wang, Jinyuan Leng, Lingling Wang, Linsheng Song

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell communication and signaling : CCS, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Wenwen Yang *Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, 52 Heishijiao Street, Dalian, 116023, China.
Jiejie Sun *Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, 52 Heishijiao Street, Dalian, 116023, China. sunjiejie@dlou.edu.cn.
Qiuyan GuoLiaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, 52 Heishijiao Street, Dalian, 116023, China.
Wei WangLiaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, 52 Heishijiao Street, Dalian, 116023, China.
Jinyuan LengLiaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, 52 Heishijiao Street, Dalian, 116023, China.
Lingling WangLiaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, 52 Heishijiao Street, Dalian, 116023, China.
Linsheng SongLiaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, 52 Heishijiao Street, Dalian, 116023, China. lshsong@dlou.edu.cn.

Funding

Dalian High Level Talent Innovation Support Program 2022RG14Dalian Outstanding Young Scientific and Technological Talent 2022RY01Liaoning Revitalization Talents Program XLYC2203087National Science Foundation of China 32222086the innovation team of Aquaculture Environment Safety from Liaoning Province LT202009
6 · The paper itself

Abstract

The leucine rich repeat and sterile alpha motif containing 1 (LRSAM1) as E3 ligase recognizes bacteria and generates a ubiquitin signal to initiate the autophagy process. In the present study, LRSAM1 was identified from the Pacific oyster Crassostrea gigas (designed as CgLRSAM1), which was able to recognize various pathogen-associated molecular patterns and bacteria and directly ubiquitinate Vibrio splendidus. V. splendidus was co-localized with CgLRSAM1 and ubiquitin after invading haemocytes, and the ubiquitinated V. splendidus was then internalized into haemocyte lysosomes by p62-LC3-mediated autophagy. In haemocytes of CgLRSAM1-RNAi oysters, the activation of CgLC3 was enhanced after V. splendidus stimulation. While the co-localization values of V. splendidus with ubiquitin, CgLC3 and lysosomes all decreased significantly after V. splendidus stimulation. These results indicated that CgLRSAM1 functioned as E3 ligase responsible for anti-Vibrio-associated ubiquitination and regulated the degradation of bacteria through the ubiquitination-autophagy-lysosome pathway.

Indexed as

CrassostreaUbiquitin-Protein LigasesAutophagyCloning, MolecularGene Expression ProfilingLysosomesPathogen-Associated Molecular Pattern MoleculesPhylogenySequence Analysis, DNAUbiquitinationPathogen-Associated Molecular Pattern MoleculesUbiquitin-Protein LigasesAutophagyBacteriaBivalvesLeucine rich repeat and sterile alpha motif containing 1Ubiquitination

Identifiers

PMID40001133
PMCPMC11863841

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.