ArticleCommunications biology2025
Propagation of goose primordial germ cells in vitro relies on FGF and BMP signalling pathways.
Article in Communications biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Short-term gonadal cultures are sufficient for germline transmission in a songbird.Stem cell reports · 2026Article
- Long-term rock dove (Columba livia) primordial germ cell culture: A tool towards avian conservation.The Journal of heredity · 2026Article
- Derivation of embryonic stem cells across avian species.Nature biotechnology · 2026Article
- Successful establishment of chicken primordial germ cell (PGC) lines from the endangered Slovakian indigenous chicken breed: the Oravka.Poultry science · 2026Article
- Review
- The chicken retrovirus-like geneiScience · 2026Article
- Article
- Efficient and Safe Knockout ofVeterinary sciences · 2026Article
- Germ cell-directed exosomal delivery enables in vivo genetic modification in birds.Journal of nanobiotechnology · 2026Article
- Transcriptome Comparison Between the Cultured and In Vivo Chick Primordial Germ Cells by SMART-Seq-Based Single-Cell RNA Sequencing.Development, growth & differentiation · 2026Article
- Advancing immunity and disease resistance in chickens through genome editing.Journal of animal science and biotechnology · 2026Review
- Analysis of Transcriptome and Differentially Expressed Genes in Chicken Primordial Germ Cells.Animals : an open access journal from MDPI · 2026Article
- Manipulating primordial germ cells from chicken and other bird species.The Journal of reproduction and development · 2026Review
- Eye pigmentation-based in-ovo chicken sexing via precision breeding.Frontiers in bioengineering and biotechnology · 2026Article
- Are we truly helping those in need: A comprehensive assessment of semen technologies in wild bird species.Biodiversity and conservation · 2026Article
- In Vitro Culture of Avian Primordial Germ Cells: Established Methods and Future Directions.Biology · 2025Review
- Genome Editing in the Chicken: From PGC-Mediated Germline Transmission to Advanced Applications.International journal of molecular sciences · 2025Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Mitotically active embryonic reproductive cells, the primordial germ cells (PGCs), are an ideal cell type for cryopreserving functional reproductive cells for avian species. Their low number in the avian embryo, however, renders cryopreservation and germline transmission methodologies difficult. Here, we develop a culture medium for the long-term in vitro culture of PGCs from the goose, Anser anser domesticus. In contrast to chicken, goose PGC self-renewal is dependent on the TGF-β family member, BMP4, and, conversely, is inhibited by Activin A. An RNA transcriptome analysis reveals commonalities between cultured PGCs from chicken and goose species, including a marked transcriptional difference between male and female goose PGCs. In vitro propagated goose PGCs are amenable to genetic modification using DNA transposons and colonising the gonads of xenogeneic sterile host embryos. These data demonstrate that the conservation and cryopreservation of the genetic diversity of the >1400 endangered bird species using PGCs remains a valid possibility.
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