Evidence map›Paper›PMID 40000394›Full record

ArticleAngewandte Chemie (International ed. in English)2025

Kinetic Redox Shotgun Proteomics Reveals Specific Lipopolysaccharide Effects on Intestinal Epithelial Cells, Mitigated by a Mn Superoxide Dismutase Mimic.

Martha Zoumpoulaki, Giovanni Chiappetta, Jean Bouvet, Namita-Raju John, Gabrielle Schanne, Pauline Gehan, Samuel Diebolt, Shakir Shakir, Elodie Quévrain, Emilie Mathieu and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Angewandte Chemie (International ed. in English), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Martha ZoumpoulakiLaboratoire Chimie Physique et Chimie du Vivant-CPCV, UMR8228, Département de Chimie, Ecole Normale Supérieure, PSL University, Sorbonne Université, CNRS, Paris, 75005, France.ORCID 0000-0002-5276-9626
Giovanni ChiappettaLaboratoire de Spectrométrie de Masse Biologique et Protéomique, SMBP, UAR2051, ESPCI Paris, PSL University, CNRS, Paris, 75005, France.ORCID 0000-0001-8959-2230
Jean BouvetLaboratoire Chimie Physique et Chimie du Vivant-CPCV, UMR8228, Département de Chimie, Ecole Normale Supérieure, PSL University, Sorbonne Université, CNRS, Paris, 75005, France.
Namita-Raju JohnLaboratoire Chimie Physique et Chimie du Vivant-CPCV, UMR8228, Département de Chimie, Ecole Normale Supérieure, PSL University, Sorbonne Université, CNRS, Paris, 75005, France.
Gabrielle SchanneLaboratoire Chimie Physique et Chimie du Vivant-CPCV, UMR8228, Département de Chimie, Ecole Normale Supérieure, PSL University, Sorbonne Université, CNRS, Paris, 75005, France.ORCID 0000-0002-8601-1485
Pauline GehanLaboratoire Chimie Physique et Chimie du Vivant-CPCV, UMR8228, Département de Chimie, Ecole Normale Supérieure, PSL University, Sorbonne Université, CNRS, Paris, 75005, France.ORCID 0009-0001-7002-6500
Samuel DieboltLaboratoire de Spectrométrie de Masse Biologique et Protéomique, SMBP, UAR2051, ESPCI Paris, PSL University, CNRS, Paris, 75005, France.
Shakir ShakirLaboratoire de Spectrométrie de Masse Biologique et Protéomique, SMBP, UAR2051, ESPCI Paris, PSL University, CNRS, Paris, 75005, France.
Elodie QuévrainLaboratoire Chimie Physique et Chimie du Vivant-CPCV, UMR8228, Département de Chimie, Ecole Normale Supérieure, PSL University, Sorbonne Université, CNRS, Paris, 75005, France.
Emilie MathieuLaboratoire Chimie Physique et Chimie du Vivant-CPCV, UMR8228, Département de Chimie, Ecole Normale Supérieure, PSL University, Sorbonne Université, CNRS, Paris, 75005, France.ORCID 0000-0002-9313-8679
Sylvie DemignotGastroenterology Department, Sorbonne Université, INSERM, Centre de Recherche Saint-Antoine, CRSA, Paris Center for Microbiome Medicine (PaCeMM) FHU, AP-HP, Saint-Antoine Hospital, Paris, France.ORCID 0000-0001-7149-1992
Philippe SeksikGastroenterology Department, Sorbonne Université, INSERM, Centre de Recherche Saint-Antoine, CRSA, Paris Center for Microbiome Medicine (PaCeMM) FHU, AP-HP, Saint-Antoine Hospital, Paris, France.ORCID 0000-0003-3596-9893
Nicolas DelsucLaboratoire Chimie Physique et Chimie du Vivant-CPCV, UMR8228, Département de Chimie, Ecole Normale Supérieure, PSL University, Sorbonne Université, CNRS, Paris, 75005, France.ORCID 0000-0001-5570-8311
Joelle VinhLaboratoire de Spectrométrie de Masse Biologique et Protéomique, SMBP, UAR2051, ESPCI Paris, PSL University, CNRS, Paris, 75005, France.ORCID 0000-0001-7184-2668
Clotilde PolicarLaboratoire Chimie Physique et Chimie du Vivant-CPCV, UMR8228, Département de Chimie, Ecole Normale Supérieure, PSL University, Sorbonne Université, CNRS, Paris, 75005, France.ORCID 0000-0003-0255-1650

Funding

Agence Nationale de la Recherche ANR-15-CE07-0027(MAGIC)Agence Nationale de la Recherche ANR-17-CONV-0005(MAIN)Agence Nationale de la Recherche ANR-20-CE07-0039(CATMAN)Agence Nationale de la Recherche ANR-21-CE18-0053(MOBIDIC)Agence Nationale de la Recherche ANR-22-PEBI-0003(PEPR)Association Francois AupetitCNRS-MITI ANACOMDACNRS-MITI BACTMANConseil Régional d'Ile-de-France Sesamen°10022268Fondation pour la Recherche Médicale DEA20151234413Indo-French Centre for the Promotion of Advanced Research CEFIPRA6505-1
6 · The paper itself

Abstract

Overproduction of reactive oxygen species and antioxidant superoxide dismutases (SOD1, SOD2) dysregulation contribute to chronic inflammation such as generated in inflammatory bowel diseases (IBD). A kinetic redox shotgun proteomic strategy (OcSILAC for Oxidized cysteine Stable Isotope Labelling by Amino acids in Cell culture) was used to explore the lipopolysaccharide (LPS) effects including LPS-induced oxidation and inflammation cascades on a dedicated intestinal epithelial cell line (HT29-MD2) together with the potential mitigating role of a Mn-based SOD-mimic Mn1. While LPS induced transient oxidative damages at early times (15 min), cells incubated with Mn1 showed, in this time frame, a significantly reduced cysteine oxidation, highlighting Mn1 antioxidant properties. Over time, cysteine oxidation of LPS-treated cells was counteracted by an overexpression of antioxidant proteins (SOD1, NQO1) and a late (6 h) preponderant increase in SOD2 level. Mn1, when co-incubated with LPS, attenuated the level of most LPS-modified proteins, that is, proteins involved in the inflammatory response. Our results highlight Mn1 as a potentially effective antioxidant and anti-inflammatory agent to consider in the treatment of IBD, as well as a useful tool for exploring the interconnection between oxidative stress and inflammation.

Indexed as

Epithelial CellsLipopolysaccharidesProteomicsSuperoxide DismutaseAntioxidantsHumansKineticsOxidation-ReductionAntioxidantsLipopolysaccharidesSuperoxide DismutaseBioinorganic chemistryLipopolysaccharide (LPS)Oxidative stressQuantitative redox proteomics OcSILACSuperoxide dismutase (SOD) mimic or mimetic

Identifiers

PMID40000394
PMCPMC12051783

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.