Soy Protein-Cultured Mesenchymal Stem Cell-Secreted Extracellular Vesicles Target the Neurovascular Unit: Insights from a Zebrafish Brain Injury Model.

Tai-I Lin, Pei-Ying Hsieh, Hui-Jen Lin, Cheng-Kang Chiang, Jim Jinn-Chyuan Sheu, Wei-Tien Chang, Ian Liau, Hsin-Yun Hsu

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Article in ACS biomaterials science & engineering, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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5 · Who and what money

Authors and funding

8 authors.

Tai-I LinDepartment of Applied Chemistry and Institute of Molecular Science, National Yang-Ming Chiao-Tung University, Hsinchu 300093, Taiwan.

Pei-Ying HsiehDepartment of Applied Chemistry and Institute of Molecular Science, National Yang-Ming Chiao-Tung University, Hsinchu 300093, Taiwan.

Hui-Jen LinDepartment of Applied Chemistry and Institute of Molecular Science, National Yang-Ming Chiao-Tung University, Hsinchu 300093, Taiwan.

Cheng-Kang ChiangDepartment of Chemistry, National Dong Hwa University, Hualien 974301, Taiwan.

Jim Jinn-Chyuan SheuInstitute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung 804201, Taiwan.

Wei-Tien ChangNational Taiwan University Hospital/National Taiwan University, Taipei 100233, Taiwan.

Ian LiauDepartment of Applied Chemistry and Institute of Molecular Science, National Yang-Ming Chiao-Tung University, Hsinchu 300093, Taiwan.ORCID 0000-0002-1373-1289

Hsin-Yun HsuDepartment of Applied Chemistry and Institute of Molecular Science, National Yang-Ming Chiao-Tung University, Hsinchu 300093, Taiwan.ORCID 0000-0001-9317-8892

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cerebral vascular disorders often accompany hypoxia-induced brain injury. In this study, we develop a zebrafish model of hypoxia-induced cerebral vascular injury to replicate the associated phenotypic changes, including cerebrovascular damage, neuronal apoptosis, and neurological dysfunction. We then explored the therapeutic potential of extracellular vesicles derived from Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) cultured on soy protein-coated surfaces. These vesicles demonstrated superior recovery efficacy, especially in restoring the blood-brain barrier integrity and improving neurological function. Our findings suggest that these potent therapeutic extracellular vesicles, easily produced from WJ-MSCs cultured in the presence of soy proteins, may mitigate hypoxia-induced brain injury by decreasing the severity of vascular disorder caused by oxidative stress. Protein-protein interactome analysis further suggests that multiple signaling pathways are likely involved in restoring normal neurovascular unit function.

Indexed as

Brain InjuriesExtracellular VesiclesMesenchymal Stem CellsSoybean ProteinsAnimalsBlood-Brain BarrierCells, CulturedDisease Models, AnimalZebrafishSoybean Proteinsextracellular vesiclesneurovascular unitsoy protein isolateszebrafish model

Identifiers

PMID40000145

DOI10.1021/acsbiomaterials.4c02304

PMCPMC11897944

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.