Evidence map›Paper›PMID 39999283›Full record

ArticleVeterinary medicine and science2025

Molecular and Seroprevalence of Mycoplasma gallisepticum in Turkeys in Sylhet District of Bangladesh.

Jahid Hasan Tipu, Rijon Miah, Obaidul Islam, Md Mukidur Rahman, Lucky Talukdar, Rubel Miah, Md Safwan Hussain, Md Ashraful Islam, Md Irtija Ahsan, Ahsan Raquib and 1 more

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Article in Veterinary medicine and science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Novel insights intomSystems · 2026
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Jahid Hasan TipuCentre for International Health, Department of Global Public Health and Primary Care, Faculty of Medicine, University of Bergen, Bergen, Norway.ORCID 0009-0001-2715-6510
Rijon MiahDepartment of Pathology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, Bangladesh.ORCID 0009-0007-1322-4854
Obaidul IslamLaboratory of Veterinary Epidemiology, College of Veterinary Medicine, Chungbuk National University, Chungbuk, South Korea.ORCID 0000-0003-3180-3706
Md Mukidur RahmanDepartment of Pathology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, Bangladesh.ORCID 0009-0007-3553-9654
Lucky TalukdarDepartment of Pathology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, Bangladesh.ORCID 0009-0008-5287-5622
Rubel MiahDepartment of Veterinary Sciences, Chonnam National University, Gwangju, South Korea.ORCID 0000-0001-5183-0643
Md Safwan HussainSchool of Public Health, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.ORCID 0000-0002-0546-4889
Md Ashraful IslamLaboratory of Veterinary Laboratory Medicine, College of Veterinary Medicine, Chungbuk National University, Chungbuk, South Korea.ORCID 0009-0004-5426-0251
Md Irtija AhsanSchool of Life Sciences, University of Warwick, Coventry, UK.ORCID 0000-0002-1761-2367
Ahsan RaquibDepartment of Health Management, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Canada.ORCID 0000-0001-5847-3239
Monira NoorDepartment of Pathology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, Bangladesh.ORCID 0000-0002-9435-113X

Funding

National Science and Technology Fellowship under the Ministry of Science and Technology (MoST), Dhaka, Bangladesh
6 · The paper itself

Abstract

Mycoplasma gallisepticum (MG) poses a significant threat to Bangladesh's poultry industry, causing substantial economic losses every year. This study aimed to determine the prevalence of MG infection in turkeys using serum plate agglutination (SPA), enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) in Sylhet, Bangladesh from December 2019 to November 2020. In addition, we evaluated the diagnostic accuracy of these tests and identified potential risk factors associated with MG infection. A total of 250 blood samples and 250 tracheal swabs were collected from suspected turkeys across 25 farms from three sub-districts of Sylhet namely Sylhet Sadar, Golapganj and Beanibazar. Blood samples were tested with SPA and ELISA, while tracheal swabs were analysed by PCR targeting the 16S rRNA gene of MG. The overall prevalence of MG was 35.2%, 29.2% and 25.6% for SPA, ELISA and PCR respectively. Higher infection rates were observed in turkeys aged 0-4 months (SPA 57.1%, ELISA 52%, PCR 42.8%), during winter (SPA 43.1%, ELISA 37.8%, PCR 30%) and among female turkeys (SPA 54.5%, ELISA 49.5%, PCR 45.5%). Geographically, the Beanibazar had the highest prevalence (SPA 54.2%, ELISA 48.6%, PCR 41.4%), compared to the Sylhet Sadar and Golapganj sub-districts. Both SPA and ELISA tests showed 100% sensitivity, with specificity of 87.1% and 95.2%, respectively using PCR as a gold standard. Overall, these findings provide valuable insights for developing effective control measures for MG infections in the poultry industry of Bangladesh.

Indexed as

Mycoplasma gallisepticumMycoplasma InfectionsPoultry DiseasesTurkeysAgglutination TestsAnimalsBangladeshEnzyme-Linked Immunosorbent AssayFemaleMalePolymerase Chain ReactionPrevalenceSeroepidemiologic Studies16S rRNA geneDiagnostic testsMycoplasma gallisepticum (MG)poultrysensitivityspecificitySylhet

Identifiers

PMID39999283
PMCPMC11855371

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.