A Platform Integrating Biophysical and Biochemical Stimuli to Enhance Differentiation and Maturation of Cardiomyocyte Subtypes Derived from Human Induced Pluripotent Stem Cells.

Zhonggang Feng, Kota Sawada, Iori Ando, Riku Yoshinari, Daisuke Sato, Tadashi Kosawada

Abstract read

In one paragraph

Article in Journal of cardiovascular development and disease, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Zhonggang FengGraduate School of Science and Engineering, Yamagata University, Yonezawa 992-8510, Japan.ORCID 0000-0002-4086-1468

Kota SawadaGraduate School of Science and Engineering, Yamagata University, Yonezawa 992-8510, Japan.

Iori AndoGraduate School of Science and Engineering, Yamagata University, Yonezawa 992-8510, Japan.

Riku YoshinariGraduate School of Science and Engineering, Yamagata University, Yonezawa 992-8510, Japan.

Daisuke SatoGraduate School of Science and Engineering, Yamagata University, Yonezawa 992-8510, Japan.ORCID 0000-0002-6923-832X

Tadashi KosawadaGraduate School of Science and Engineering, Yamagata University, Yonezawa 992-8510, Japan.

Funding

Japan Society for the Promotion of Science 21K12661

6 · The paper itself

Abstract

To enhance the differentiation and maturation of cardiomyocytes derived from human induced pluripotent stem cells, we developed a bioreactor system that simultaneously imposes biophysical and biochemical stimuli on these committed cardiomyocytes. The cells were cultured within biohydrogels composed of the extracellular matrix extracted from goat ventricles and purchased rat-origin collagen, which were housed in the elastic PDMS culture chambers of the bioreactor. Elastic and flexible electrodes composed of PEDOT/PSS, latex, and graphene flakes were embedded in the hydrogels and chamber walls, allowing cyclic stretch and electrical pulses to be simultaneously and coordinately applied to the cultured cells. Furthermore, a dynamic analysis method employing the transverse forced oscillation theory of a cantilever was used to analyze and discriminate the subtype-specific beating behavior of the cardiomyocytes. It was found that myosin light chain 2v (

Indexed as

bioreactorcardiac differentiationcardiomyocyte subtypesmechano-electrical stimulationsubtype aggregationthree-dimensional cultureventricular extracellular matrix

Identifiers

PMID39997490

DOI10.3390/jcdd12020056

PMCPMC11856794

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.