Evidence map›Paper›PMID 39994786›Full record

ArticleFluids and barriers of the CNS2025

Ependymal cilia decline and AQP4 upregulation in young adult rats with syringomyelia.

Longbing Ma, Sumei Liu, Qingyu Yao, Xinyu Wang, Jian Guan, Fengzeng Jian

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Article in Fluids and barriers of the CNS, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

6 authors.

Longbing Ma *Department of Neurosurgery, China International Neuroscience Institute, Xuanwu Hospital Capital Medical University, 45 Changchun Street, Beijing, 100053, China.
Sumei Liu *Department of Neurosurgery, China International Neuroscience Institute, Xuanwu Hospital Capital Medical University, 45 Changchun Street, Beijing, 100053, China.
Qingyu YaoDepartment of Neurosurgery, China International Neuroscience Institute, Xuanwu Hospital Capital Medical University, 45 Changchun Street, Beijing, 100053, China.
Xinyu WangDepartment of Neurosurgery, China International Neuroscience Institute, Xuanwu Hospital Capital Medical University, 45 Changchun Street, Beijing, 100053, China.
Jian GuanDepartment of Neurosurgery, China International Neuroscience Institute, Xuanwu Hospital Capital Medical University, 45 Changchun Street, Beijing, 100053, China.
Fengzeng JianDepartment of Neurosurgery, China International Neuroscience Institute, Xuanwu Hospital Capital Medical University, 45 Changchun Street, Beijing, 100053, China. jianfengzeng@xwh.ccmu.edu.cn.

Funding

Beijing Natural Science Foundation L212007
6 · The paper itself

Abstract

backgroundSyringomyelia was a rare condition characterized by the formation of fluid-filled cysts (syrinx) within the spinal cord, resulting in sensory and motor dysfunction. Currently, there was no satisfactory treatment for syringomyelia. Ependymal cells were integral to water transport and may represent a promising therapeutic target.

methodsInduction of syringomyelia occurred in 8-week old female rats followed by histological analyses at 3-, 7-, 14-, 30-, 60-, 180-, and 365-days later. Scanning electron microscope (SEM) and transmission electron microscope (TEM) were performed to visualize cilia on rat central canal membrane cells at 30-day post-induction. Syringomyelia was induced via compression at T12-T13 using a sterile cotton ball. Each rat underwent MRI scanning one day before induction and one day prior to sacrifice. In vivo magnetic resonance imaging (MRI) was utilized to measure syrinx enlargement in eight-week-old syringomyelia rats. Histological Analysis and immunofluorescence staining were performed for changes of cilia, neurons, expression of AQP4 and infiltration of immune cells into spinal tissue.

resultsIn the current study, the cell junctions between ependymal cells of syringomyelia rats were absent, and the cilia on ependymal cells were reduced significantly on day 30 post syringomyelia. The number of ependymal cells kept increasing lasting for 1-2 months and begin to decrease. Edema and vacuolation in the spinal cord tissue are significant in syringomyelia rats. Furthermore, AQP4 expression was elevated in astrocytes of syringomyelia rats, and IBA1

conclusionsThese findings underscored the significance of cilia on ependymal cells and the evolving microenvironment post-syringomyelia, providing valuable insights for clinical treatment strategies for this condition.

Indexed as

Aquaporin 4CiliaEpendymaSyringomyeliaAnimalsDisease Models, AnimalFemaleMagnetic Resonance ImagingRatsRats, Sprague-DawleySpinal CordUp-RegulationAqp4 protein, ratAquaporin 4CiliaEpendymal cellNeuronal necrosisRatSpinal cord injurySyringomyelia

Identifiers

PMID39994786
PMCPMC11849276

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