ArticleParasites & vectors2025
Giardiavirus infection alleviates growth restriction and intestinal damage caused by the intestinal parasite Giardia duodenalis.
Article in Parasites & vectors, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed.
- Giardiavirus rewires host translation and glycolytic metabolism to support its replication inVirulence · 2026Article
- Expanding the Antimicrobial Toolbox with Therapeutic Viruses: Mechanisms, Pharmaceutical Formulation, and Translational Outlook.Pharmaceuticals (Basel, Switzerland) · 2026Review
- Interactions between extracellular vesicles and viruses: lessons learned across species and kingdoms.FEMS microbiology reviews · 2026Review
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Authors and funding
13 authors.
Funding
Abstract
backgroundGiardia duodenalis is a prevalent intestinal pathogen causing giardiasis, a condition characterized by diarrhea and frequently linked to malnutrition and growth impairments in children. The virulence of Giardiavirus (GLV) may efficiently clear Giardia parasites from infected patients. However, we have a limited understanding of GLV transmission among Giardia species and GLV-infected Giardia's impact on pathogenicity.
methodsThis study investigated extracellular vesicles (EVs) isolated via ultracentrifugation or exosome assay kit to detect the presence of GLV in EVs, the results were detected using ultrastructure and molecular methods, including transmission electron microscopy, scanning electron microscopy, quantitative polymerase chain reaction (qPCR), and dot blot. Transwell migration assays confirmed the spread of GLV-enveloped EVs among Giardia species using inhibitor experiments and immunofluorescence. Mice gavaged with Giardia, with or without GLV infection, were assessed for disease progression, including growth parameters (weight and size gains), intestinal permeability, and pathology.
resultsParts of GLV exploit the Giardia EVs pathway to reach the extracellular environment, allowing GLV to spread among Giardia species via these EVs. The uptake of GLV-containing EVs by Giardia results in rapid trophozoite infection, and GLV wrapped in EVs also offers protection against external interference. Importantly, EV-coated GLV-infected Giardia leads to divergent clinical symptoms in mice, posing less risk to mice and reducing symptoms, such as emaciation, stunted growth, and lesion damage, compared with GLV-free Giardia-infected mice.
conclusionsOur studies show that GLV wrapped in EVs can spread among Giardia species, and GLV infection alleviates the lesions caused by Giardia. These findings reveal that GLV could be a target for the development of novel intervention strategies against Giardia.
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