Evidence map›Paper›PMID 39994360›Full record

ArticleScientific reports2025

Isolation and characterization of new lytic bacteriophage PSA-KC1 against Pseudomonas aeruginosa isolates from cystic fibrosis patients.

Kübra Can Kurt, Halil Kurt, Edip Tokuç, Doğukan Özbey, Duygu Nur Arabacı, Sevcan Aydın, Nevriye Gönüllü, Mikael Skurnik, Hrisi Bahar Tokman

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PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Review
  6. Article
  7. Review
  8. Review
  9. Characterization and Genomic Analysis of a New BacteriophageAntibiotics (Basel, Switzerland) · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Kübra Can KurtHamidiye Faculty of Dentistry, Department of Basic Medical Sciences, University of Health Sciences, Istanbul, Türkiye. kubra.cankurt@sbu.edu.tr.ORCID http://orcid.org/0000-0001-6862-7908
Halil KurtMedical Biology Department, University of Health Sciences, Hamidiye International School of Medicine, Istanbul, Türkiye, Turkey.ORCID http://orcid.org/0000-0002-7392-527X
Edip TokuçCerrahpasa Medical Faculty, Medical Microbiology Department, İstanbul University-Cerrahpasa, 34320, Istanbul, Türkiye, Turkey.ORCID http://orcid.org/0000-0002-2440-3125
Doğukan ÖzbeyFaculty of Medicine, Medical Microbiology Department, Istanbul Okan University, Istanbul, Türkiye, Turkey.ORCID http://orcid.org/0000-0002-0596-1551
Duygu Nur ArabacıDepartment of Genetics and Bioengineering, Nişantaşı University, Istanbul, Türkiye, Turkey.ORCID http://orcid.org/0000-0002-6505-7986
Sevcan AydınFaculty of Science, Department of Biology, Biotechnology Section, Istanbul University, Istanbul, Türkiye, Turkey.ORCID http://orcid.org/0000-0002-8935-7932
Nevriye GönüllüCerrahpasa Medical Faculty, Medical Microbiology Department, İstanbul University-Cerrahpasa, 34320, Istanbul, Türkiye, Turkey.ORCID http://orcid.org/0000-0003-4289-1975
Mikael SkurnikDepartment of Bacteriology and Immunology, Human Microbiome Research Program, Faculty of Medicine, University of Helsinki, Helsinki, 00290, Finland.ORCID https://orcid.org/0000-0001-8791-9260
Hrisi Bahar TokmanCerrahpasa Medical Faculty, Medical Microbiology Department, İstanbul University-Cerrahpasa, 34320, Istanbul, Türkiye, Turkey.ORCID https://orcid.org/0000-0002-2205-5120

Funding

Istanbul University-Cerrahpasa, BAP IUC-BAP 34517
6 · The paper itself

Abstract

A novel lytic bacteriophage, PSA-KC1, was isolated from wastewater. In this study, the whole genome of the bacteriophage PSA-KC1 was analyzed, and its lytic properties were assessed. PSA-KC1 has a linear double-stranded DNA genome with a total length of 43,237 base pairs and a GC content of 53.6%. In total, 65 genes were predicted, 46 of which were assigned functions as structural proteins involved in genome replication, packaging or phage lysis. PSA-KC1 belongs to the genus Septimatrevirus under the Caudoviricetes class. The aim of this study was to investigate the efficacy of the lytic bacteriophage PSA-KC1 and compare it with that of the Pyophage phage cocktail on 25 multi drug resistant (MDR) Pseudomonas aeruginosa strains isolated from sputum samples of cystic fibrosis patients. Seventeen of these strains were susceptible (68%) to the PSA-KC1 lytic phage we isolated, whereas eight clinical strains were resistant. However, 22 (88%) of the P. aeruginosa strains were susceptible to the Pyophage cocktail, and three (12%) were resistant to the Phage cocktail. At the end of our study, a new lytic phage active against multidrug-resistant P. aeruginosa strains from CF patients was isolated, and its genome was characterized. Since the PSA-KC1 phage does not contain virulence factors, toxins or integrase genes, it can be expected to be a therapeutic candidate with the potential to be used safely in phage therapy.

Indexed as

BacteriophagesCystic FibrosisPseudomonas aeruginosaPseudomonas InfectionsPseudomonas PhagesDrug Resistance, Multiple, BacterialGenome, ViralHumansAntibiotic resistanceCystic fibrosisPhage therapyPseudomonas aeruginosaWhole-genome sequencing

Identifiers

PMID39994360
PMCPMC11850609

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.