ArticleScientific reports2025
Pt-LIS1 participates nuclear deformation and acrosome formation via regulating Dynein-1 during spermatogenesis in Portunus trituberculatus.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Spermatogenesis involves complex dynamic mechanisms. Dynein-1 is a key carrier in cellular cargo transport, participating in nuclear deformation and acrosome formation during spermatogenesis. However, the regulatory mechanisms of Dynein-1 during cargo transport remain unknown. In this study, we explored the role of Lissencephaly 1 (LIS1) in spermatogenesis and its impact on Dynein-1 cargo transport in Portunus trituberculatus. LIS1 was known as a Dynein-1 regulator, which is a causative gene of anencephaly syndrome. Pt-Lis1 was cloned from the crab testis, and its highly expression was observed in the testis. Pt-LIS1 dynamically localized around the nucleus and acrosome during spermatogenesis, colocalizing with Dynein-1 subunits, microtubules, mitochondrial markers (PHB), and Acrosin. RNA interference reduced Pt-Lis1 expression, leading to decreased expression of Pt-dhc and Pt-dic. During spermatogenesis, the signals of Pt-LIS1, Pt-DHC, Pt-DIC, and α-Tubulin were weakened and showed disorganized distribution. The colocalization of Pt-LIS1 with Pt-DHC and Pt-DIC decreased, while abnormal colocalization significantly increased. In addition, caspase-3 and p53 expression significantly increased after Pt-Lis1 silencing, indicating association with apoptosis in spermatogenic cells. All these results suggest that LIS1 played a crucial role in crustacean spermatogenesis by regulating nuclear deformation and acrosome formation through modulating Dynein-1 transport cargoes along microtubules.
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