Evidence map›Paper›PMID 39993695›Full record

ArticleClinical immunology (Orlando, Fla.)2025

Spatial transcriptomics of Glomerulo-centric antibody mediated rejection in renal transplants.

Dajana Margeta, Hirotsugu Noguchi, Sepideh Khazaie, Leal C Herlitz, Joshua J Augustine, Peter S Heeger, Anat R Tambur, Robert L Fairchild, William M Baldwin

Abstract readCase Reports
PubMed Full text at PMC Publisher
In one paragraph

Article in Clinical immunology (Orlando, Fla.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Dajana MargetaDepartment of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic, OH, USA.
Hirotsugu NoguchiDepartment of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic, OH, USA.
Sepideh KhazaieDepartment of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic, OH, USA.
Leal C HerlitzDepartment of Anatomic Pathology, Cleveland Clinic, OH, USA.
Joshua J AugustineDepartment of Nephrology and Hypertension, Cleveland Clinic, OH, USA.
Peter S HeegerComprehensive Transplant Center, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Anat R TamburDepartment of Surgery, Comprehensive Transplant Center, Northwestern University, Chicago, IL, USA.
Robert L FairchildDepartment of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic, OH, USA.
William M BaldwinDepartment of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic, OH, USA. Electronic address: baldwiw@ccf.org.

Funding

Noninvasive Markers and Transplant Outcome in HumansU01AI063594 · NIAID · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI HEEGER, PETER SCOTT · 2004 to 2022
$66.5M
POSTTRANSPLANT INFLAMMATORY RESPONSER01AI040459 · NIAID · CLEVELAND CLINIC LERNER COM-CWRU · PI Robert L Fairchild · 1997 to 2026
$9.4M
Complement regulates macrophage and platelet function in kidney transplantsR01AI165513 · NIAID · CLEVELAND CLINIC LERNER COM-CWRU · PI BALDWIN, WILLIAM M · 2021 to 2025
$3.1M
Acute Antibody Mediated Kidney Allograft RejectionR01AI158421 · NIAID · CLEVELAND CLINIC LERNER COM-CWRU · PI FAIRCHILD, ROBERT L · 2021 to 2025
$3.0M
NIAID NIH HHS R01 AI040459NIAID NIH HHS R01 AI158421NIAID NIH HHS R01 AI165513NIAID NIH HHS U01 AI063594
6 · The paper itself

Abstract

In this case study, we used Digital Spatial Profiling to localize transcripts in a series of 4 biopsies from a single patient before, during and after treatment for acute antibody-mediated rejection that was characterized by strong C4d staining of the glomeruli. Spatial resolution demonstrated that molecular signatures of innate immune cells including NK cells and macrophages are located in glomeruli during AMR, and transcripts for HLA class II antigens were upregulated in the glomeruli. In contrast, transcripts of signature genes for podocytes were decreased during rejection. Treatment with IVIg resolved histological evidence of glomerulitis but did not restore expression of podocyte transcripts. These data demonstrate a vulnerability of podocytes in acute AMR with persistent glomerulitis. Additionally, by using a protocol biopsy from the same patient as a baseline, transcript changes for an informative set of genes were uncovered to test for podocyte dysfunction in future patients.

Indexed as

Graft RejectionKidney GlomerulusKidney TransplantationTranscriptomeAdultBiopsyComplement C4bFemaleGene Expression ProfilingHumansImmunoglobulins, IntravenousKiller Cells, NaturalMaleMiddle AgedPodocytesComplement C4bImmunoglobulins, IntravenousAntibody-mediated rejectionC4d stainingDigital spatial profilingHLA class II antigensMacrophagesPodocytes

Identifiers

PMID39993695
PMCPMC12151301

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.