ArticleMethods in molecular biology (Clifton, N.J.)2025
Measurement of Neurotransmitters and Metabolites in Cell Culture and In Vitro Using Genetically Encoded PBP-Based Biosensors.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed.
- Microglia coordinate activity-dependent protein synthesis in neurons through metabolic coupling.bioRxiv : the preprint server for biology · 2025Article
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1 author.
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Abstract
Some proteins undergo conformational changes when they bind their cognate ligand. This conformational change can be coupled to changes in the local environment of the chromophore of green fluorescent protein (GFP) to create genetically encoded sensors for those ligands. The periplasmic binding proteins of bacteria bind diverse classes of molecules and have been made into sensors for a number of neurotransmitters and metabolites. Here we describe protocols for using these sensors to quantify analytes in complex solutions in vitro and how to use them in cell culture-based assays to observe fluctuations in their concentration over time, from the millisecond existence of neurotransmitters to the consumption of cytosolic metabolites over the course of hours.
Indexed as
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39992515What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.