ArticleArchives of microbiology2025
Development and evaluation of a novel rapid fungal nucleic acid extraction kit.
Article in Archives of microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Research progress on nucleic acid amplification-based detection technologies for phytopathogenic fungi.Applied microbiology and biotechnology · 2026Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fungal infections can cause different diseases, and nucleic acid extraction is a crucial step in assisting the diagnosis of fungal infections. Traditional methods and current commercial fungal nucleic acid extraction kits are often time-consuming and involve hazardous chemicals. We developed a nucleic acid extraction kit that is safe, rapid, and efficient, designated as FNA-REK (Fungal Nucleic Acid Extraction Kit), which facilitates the simultaneous extraction of DNA and RNA without the necessity for hazardous chemicals, liquid nitrogen, or glass beads. This kit is user-friendly and achieves complete extraction within a mere 10 min. The RNA from the culture of Cryptococcus neoformans, Candida albicans, Candida krusei, Aspergillus flavus, Aspergillus niger and Aspergillus fumigatus. Meanwhile, the RNA from Candida albicans, Candida glabrata and Candida tropicalis of mid-stream urine, vaginal discharge and sputum samples, respectively, Cryptococcus neoformans, genus Aspergillus and Pneumocystis jirovecii of sputum samples, genus Aspergillus of bronchoscopy rinsing fluid were extracted using both the FNA-REK and the BIO-TEK. The concentration and purity of the extracted RNA were measured by nanodrop photometer. The nucleic acid extracted from Cryptococcus neoformans and Candida albicans using FNA-REK showed higher concentration and purity compared to BIO-TEK following a 10
Indexed as
Identifiers
39992430What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.