Evidence map›Paper›PMID 39992125›Full record

ArticlemBio2025

Using fluorescently labeled wheat germ agglutinin to track lipopolysaccharide transport to the outer membrane in

Laurent Dubois, Andrea Vettiger, Jackson A Buss, Thomas G Bernhardt

Abstract read
In one paragraph

Article in mBio, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. The mycomembrane proteins PorH and ProtX are inserted at polar growth zones and linked to the cell wall.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  2. Article
  3. Article
  4. Tissue-like structures formed by a bacterium.bioRxiv : the preprint server for biology · 2025
    Article
  5. Autonomous division of the outer membrane in Gram-negative bacteria.bioRxiv : the preprint server for biology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Laurent DuboisDepartment of Microbiology, Harvard Medical School, Boston, Massachusetts, USA.
Andrea VettigerDepartment of Microbiology, Harvard Medical School, Boston, Massachusetts, USA.
Jackson A BussDepartment of Microbiology, Harvard Medical School, Boston, Massachusetts, USA.
Thomas G BernhardtDepartment of Microbiology, Harvard Medical School, Boston, Massachusetts, USA.ORCID 0000-0003-3566-7756

Funding

Project 3: Defining and defeating the mechanisms of outer membrane biogenesis in Gram-negative bacteriaU19AI158028 · NIAID · HARVARD MEDICAL SCHOOL · PI WALKER, SUZANNE · 2022 to 2025
$10.1M
Peptidoglycan Biogenesis in Escherichia ColiR01AI083365 · NIAID · HARVARD MEDICAL SCHOOL · PI Thomas G Bernhardt · 2010 to 2026
$8.3M
NIAID NIH HHS R01 AI083365NIAID NIH HHS U19 AI158028
6 · The paper itself

Abstract

The cell envelope of gram-negative bacteria consists of two membranes sandwiching the peptidoglycan (PG) cell wall. The outer membrane (OM) contains integrated beta-barrel proteins and has an outer leaflet composed of lipopolysaccharide (LPS). LPS is transported from the inner membrane where it is made to the OM surface by the Lpt system. In the polarly elongating alpha-proteobacterium

Indexed as

Bacterial Outer MembraneEscherichia coliFluorescent DyesLipopolysaccharidesWheat Germ AgglutininsBacterial Outer Membrane ProteinsBiological TransportCell MembraneStaining and LabelingBacterial Outer Membrane ProteinsFluorescent DyesLipopolysaccharidesWheat Germ Agglutininslipopolysaccharideouter membranewheat germ agglutinin

Identifiers

PMID39992125
PMCPMC11898776

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.