ArticleHLA2025
Four Target Resequencing for the Bovine Major Histocompatibility Complex Region. Proof of Concept.
Article in HLA, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
The bovine leukocyte antigen (BoLA) comprises four regions that contain a high density of polymorphic genes and frequently show gene copy number variations (CNV). Therefore, genotyping BoLA using genome-wide resequencing is difficult. This study aimed to develop four probe sets for resequencing of the BoLA region using a hybridization capture target next-generation sequencing (NGS) method. This proof of concept showed and discussed the several applications of the used strategy. DNAs from nine Japanese Black cows and one Holstein cow were genotyped for BoLA-DRB3 using PCR sequence-based typing (SBT). DNA libraries were constructed using the KAPA HyperPlus Kit, and BoLA DNA sequences were enriched using the SeqCap EZ kit and four custom-made probes. Based on preliminary results, the probe set BoLA2 was selected for further analysis. This analysis resulted in a mean coverage of 90.8% with an average depth of 108 reads. A total of 113,646 SNPs and 17,995 indels were detected, several of which have previously been described in the dbSNP database. This allowed the genotyping of class II genes, including BoLA-DRB3. A comparison between target resequencing and PCR-SBT assays did not show conflicts between the BoLA-DRB3 genotyping results. CNV analysis based on read number inferred that the BoLA-DQA1, BoLA-DQA2, BoLA-DQA5, and BoLA-DQB genes would be present in the homozygous or heterozygous states or absent, allowing for the definition of four class II and three class I haplotypes. In addition, CNV of non-classical class I genes were also observed. In conclusion, results show that approach used in this study is a cost-effective strategy for sequencing large samples for many research purposes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.