Evidence map›Paper›PMID 39990443›Full record

ArticlebioRxiv : the preprint server for biology2025

Structure of endogenous Pfs230:Pfs48/45 in complex with potent malaria transmission-blocking antibodies.

Ezra T Bekkering, Randy Yoo, Sophia Hailemariam, Fabian Heide, Danton Ivanochko, Matthew Jackman, Nicholas I Proellochs, Rianne Stoter, Geert-Jan van Gemert, Ayana Maeda and 13 more

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PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

23 authors.

Ezra T BekkeringDepartment of Medical Microbiology, Radboud University Medical Center, The Netherlands.
Randy YooProgram in Molecular Medicine, The Hospital for Sick Children Research Institute, Toronto, ON, Canada.ORCID 0000-0002-6952-9039
Sophia HailemariamProgram in Molecular Medicine, The Hospital for Sick Children Research Institute, Toronto, ON, Canada.
Fabian HeideProgram in Molecular Medicine, The Hospital for Sick Children Research Institute, Toronto, ON, Canada.
Danton IvanochkoProgram in Molecular Medicine, The Hospital for Sick Children Research Institute, Toronto, ON, Canada.
Matthew JackmanProgram in Molecular Medicine, The Hospital for Sick Children Research Institute, Toronto, ON, Canada.
Nicholas I ProellochsDepartment of Medical Microbiology, Radboud University Medical Center, The Netherlands.
Rianne StoterDepartment of Medical Microbiology, Radboud University Medical Center, The Netherlands.
Geert-Jan van GemertDepartment of Medical Microbiology, Radboud University Medical Center, The Netherlands.
Ayana MaedaDivision of Malaria Research, Proteo-Science Center, Ehime University, Matsuyama, Japan.
Takaaki YuguchiDivision of Malaria Research, Proteo-Science Center, Ehime University, Matsuyama, Japan.
Oscar T WandersDepartment of Medical Microbiology, Radboud University Medical Center, The Netherlands.
Renate C van DaalenDepartment of Medical Microbiology, Radboud University Medical Center, The Netherlands.
Maartje R InklaarDepartment of Medical Microbiology, Radboud University Medical Center, The Netherlands.
Carolina M AndradeDepartment of Medical Microbiology, Radboud University Medical Center, The Netherlands.
Pascal W T C JansenDepartment of Molecular Biology, Faculty of Science, Oncode Institute, Radboud University Nijmegen, Nijmegen, The Netherlands.
Michiel VermeulenDepartment of Molecular Biology, Faculty of Science, Oncode Institute, Radboud University Nijmegen, Nijmegen, The Netherlands.
Teun BousemaDepartment of Medical Microbiology, Radboud University Medical Center, The Netherlands.ORCID 0000-0003-2666-094X
Eizo TakashimaDivision of Malaria Research, Proteo-Science Center, Ehime University, Matsuyama, Japan.
John L RubinsteinProgram in Molecular Medicine, The Hospital for Sick Children Research Institute, Toronto, ON, Canada.ORCID 0000-0003-0566-2209
Taco W A KooijDepartment of Medical Microbiology, Radboud University Medical Center, The Netherlands.ORCID 0000-0002-8547-7523
Matthijs M JoreDepartment of Medical Microbiology, Radboud University Medical Center, The Netherlands.
Jean-Philippe JulienProgram in Molecular Medicine, The Hospital for Sick Children Research Institute, Toronto, ON, Canada.ORCID 0000-0001-7602-3995

Funding

ChimeraX -- Next Generation Visualization and Analysis Software for Multiscale ModelingR01GM129325 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI FERRIN, THOMAS E · 2018 to 2025
$5.2M
NIGMS NIH HHS R01 GM129325
6 · The paper itself

Abstract

The Pfs230:Pfs48/45 complex forms the basis for leading malaria transmission-blocking vaccine candidates, yet little is known about its molecular assembly. Here, we used cryogenic electron microscopy to elucidate the structure of the endogenous Pfs230:Pfs48/45 complex bound to six potent transmission-blocking antibodies. Pfs230 consists of multiple domain clusters rigidified by interactions mediated through insertion domains. Membrane-anchored Pfs48/45 forms a disc-like structure and interacts with a short C-terminal peptide on Pfs230 that is critical for Pfs230 membrane-retention

Indexed as

cryo-electron microscopyMalariamonoclonal antibodiesPfs230Pfs48/45Plasmodium falciparumtransmission-blocking vaccinestransmission-reducing activity

Identifiers

PMID39990443
PMCPMC11844449

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.