Evidence map›Paper›PMID 39990390›Full record

ArticlebioRxiv : the preprint server for biology2025

Epsin1 enforces a condensation-dependent checkpoint for ubiquitylated cargo during clathrin-mediated endocytosis.

Susovan Sarkar, Hao-Yang Liu, Feng Yuan, Brandon T Malady, Liping Wang, Jessica Perez, Eileen M Lafer, Jon M Huibregtse, Jeanne C Stachowiak

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Susovan SarkarDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, TX, United States.
Hao-Yang LiuDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, TX, United States.
Feng YuanDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, TX, United States.
Brandon T MaladyDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, TX, United States.
Liping WangDepartment of Biochemistry and Structural Biology, The University of Texas Health Science Center at San Antonio, San Antonio, TX, United States.
Jessica PerezDepartment of Molecular Biosciences, The University of Texas at Austin, Austin, TX, United States.
Eileen M LaferDepartment of Biochemistry and Structural Biology, The University of Texas Health Science Center at San Antonio, San Antonio, TX, United States.
Jon M HuibregtseDepartment of Molecular Biosciences, The University of Texas at Austin, Austin, TX, United States.
Jeanne C StachowiakDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, TX, United States.ORCID 0000-0003-2501-142X

Funding

Protein Networks as Synergistic Drivers of Membrane RemodelingR35GM139531 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI Jeanne Casstevens Stachowiak · 2021 to 2026
$4.0M
NIGMS NIH HHS R35 GM139531
6 · The paper itself

Abstract

Clathrin-mediated endocytosis internalizes proteins and lipids from the cell surface, supporting nutrient uptake, signaling, and membrane trafficking. Recent work has demonstrated that a flexible, liquid-like network of initiator proteins is responsible for catalyzing assembly of clathrin-coated vesicles in diverse organisms including yeast, mammals, and plants. How do cells regulate the assembly of this dynamic network to produce cargo-loaded vesicles? Here we reveal the ability of an endocytic adaptor protein, Epsin1, to conditionally stabilize the initiator protein network, creating a cargo-dependent checkpoint during clathrin-mediated endocytosis. Epsin1 is known to recruit ubiquitylated transmembrane proteins to endocytic sites. Using

Identifiers

PMID39990390
PMCPMC11844442

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.