Evidence map›Paper›PMID 39989598›Full record

ArticleNanotechnology, science and applications2025

Nanoparticles in Plant Cryopreservation: Effects on Genetic Stability, Metabolic Profiles, and Structural Integrity in Bleeding Heart (Papaveraceae) Cultivars.

Dariusz Kulus, Alicja Tymoszuk, Alicja Kulpińska, Bożena Dębska, Agata Michalska, Julita Nowakowska, Dorota Wichrowska, Jacek Wojnarowicz, Urszula Szałaj

Abstract read
In one paragraph

Article in Nanotechnology, science and applications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Genetic Stability and Photosystem II Functioning of In Vitro-RecoveredInternational journal of molecular sciences · 2025
    Article
  5. Article
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Dariusz KulusLaboratory of Horticulture, Department of Biotechnology, Faculty of Agriculture and Biotechnology, Bydgoszcz University of Science and Technology, Bydgoszcz, Poland.ORCID 0000-0001-5826-6950
Alicja TymoszukLaboratory of Horticulture, Department of Biotechnology, Faculty of Agriculture and Biotechnology, Bydgoszcz University of Science and Technology, Bydgoszcz, Poland.ORCID 0000-0001-5399-9530
Alicja KulpińskaLaboratory of Horticulture, Department of Biotechnology, Faculty of Agriculture and Biotechnology, Bydgoszcz University of Science and Technology, Bydgoszcz, Poland.
Bożena DębskaDepartment of Biogeochemistry and Soil Science, Faculty of Agriculture and Biotechnology, Bydgoszcz University of Science and Technology, Bydgoszcz, Poland.
Agata MichalskaDepartment of Biogeochemistry and Soil Science, Faculty of Agriculture and Biotechnology, Bydgoszcz University of Science and Technology, Bydgoszcz, Poland.
Julita NowakowskaImaging Laboratory, Faculty of Biology, University of Warsaw, Warsaw, Poland.
Dorota WichrowskaDepartment of Microbiology and Food Technology, Faculty of Agriculture and Biotechnology, Bydgoszcz University of Science and Technology, Bydgoszcz, Poland.ORCID 0000-0003-0037-2309
Jacek WojnarowiczLaboratory of Nanostructures, Institute of High Pressure Physics, Polish Academy of Sciences, Warsaw, Poland.ORCID 0000-0002-6106-5188
Urszula SzałajLaboratory of Nanostructures, Institute of High Pressure Physics, Polish Academy of Sciences, Warsaw, Poland.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Purpose: Studying the role of nanoparticles in plant cryopreservation is essential for developing innovative methods to conserve plant genetic resources amid environmental challenges. This research investigated the effects of gold (AuNPs), silver (AgNPs), and zinc oxide (ZnONPs) nanoparticles on the structural integrity, genetic stability, and metabolic activity of cryopreserved plant materials with medicinal properties. Methods: Shoot tips from two bleeding heart ( Results: Electron microscopy observations of LN-derived plant material confirmed the production of micro-morpho-structurally stable cells. It was found that nanoparticles could penetrate the cell and accumulate in its various compartments, including the nucleus. As for the genetic analysis, SCoT markers identified polymorphisms in 11.5% of 'Gold Heart' plants, while RAPDs detected mutations in 1.9% of 'Valentine' specimens. Analysis of Molecular Variance (AMOVA) indicated that in the 'Valentine' cultivar, all genetic variation detected was within populations and not significantly affected by nanoparticle treatments. In 'Gold Heart', the majority (94%) of genetic variation detected was within populations, while 6% was attributed to nanoparticle treatments (mostly the application of 15 ppm ZnONPs). The application of nanoparticles significantly influenced the metabolic profile of bleeding heart plants, particularly affecting the synthesis of phenolic acids and aldehydes, as well as the antioxidant mechanisms in both 'Gold Heart' and 'Valentine' cultivars. The content of proteins was altered in 'Gold Heart' plants but not in 'Valentine'. Conclusion: The results suggest that different types and concentrations of NPs have varying effects on the production of specific metabolites, which could be harnessed to modulate plant secondary metabolism for desired pharmacological outcomes.

Indexed as

antioxidant capacitygenotypingHPLCLamprocapnos spectabilis (L.) Fukuharaphenolic acidsphenylpropanoid pathwayTEM

Identifiers

PMID39989598
PMCPMC11844321

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.