Evidence map›Paper›PMID 39988994›Full record

ArticleLipids2025

A miniaturized iodine value assay for quantifying the unsaturated fatty acid content of lipids, lipid mixtures, and biological membranes.

Maria Monserrat Roman-Lara, Katie J Chong, Roslyn M Bill, Alan D Goddard

Abstract read
In one paragraph

Article in Lipids, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Maria Monserrat Roman-LaraAston Institute for Membrane Excellence and School of Biosciences, College of Health and Life Sciences, Aston University, Birmingham, UK.
Katie J ChongEnergy and Bioproducts Research Institute, College of Engineering and Physical Sciences, Birmingham, UK.
Roslyn M BillAston Institute for Membrane Excellence and School of Biosciences, College of Health and Life Sciences, Aston University, Birmingham, UK.
Alan D GoddardAston Institute for Membrane Excellence and School of Biosciences, College of Health and Life Sciences, Aston University, Birmingham, UK.ORCID 0000-0003-4950-7470

Funding

Biotechnology and Biological Sciences Research Council BB/R02152X/1Consejo Nacional de Ciencia y Tecnología 2018-000024-01EXTF-00053Research England
6 · The paper itself

Abstract

Various methods exist for identifying and quantifying lipid unsaturation, including mass spectrometry and Raman spectroscopy. A disadvantage of these existing approaches is the need for sophisticated equipment and software, placing them beyond the means of many laboratories. The iodine value (IV) is a colorimetric unsaturation index; however, it uses iodine monochloride, a hazardous chemical, and considerable amounts of sample. Here, we demonstrate the first use of a miniaturized IV method that requires only milliliter quantities of hazardous chemicals and sample sizes such that it is feasible to assay biological membranes. Briefly, lipids are exposed to iodine monochloride, resulting in the replacement of unsaturated bonds with di-halogenated single bonds. Potassium iodide then reacts with unreacted iodine monochloride forming I

Indexed as

Cell MembraneFatty Acids, UnsaturatedIodineLipidsMembrane LipidsEscherichia coliFatty Acids, UnsaturatedIodineLipidsMembrane Lipidscell membranecolorimetryIodine Valuelipid unsaturationWijs reagent

Identifiers

PMID39988994
PMCPMC12235214

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.