Evidence map›Paper›PMID 39988668›Full record

ArticleBMC plant biology2025

BAP regulates lateral bud outgrowth to promote tillering in Paphiopedilum callosum (Orchidaceae).

Yuying Yin, Ronghui Zhong, Yefei Li, Beiyi Guo, Lin Li, Guohua Ma, Kunlin Wu, Lin Fang, Songjun Zeng

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Deciphering Molecular Pathways ofMicroorganisms · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yuying YinGuangdong Provincial Key Laboratory of Applied Botany and Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China.
Ronghui ZhongGuangdong Key Laboratory of Ornamental Plant Germplasm Innovation and Utilization, Environmental Horticulture Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
Yefei LiGuangdong Provincial Key Laboratory of Applied Botany and Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China.
Beiyi GuoGuangdong Provincial Key Laboratory of Applied Botany and Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China.
Lin LiGuangdong Provincial Key Laboratory of Applied Botany and Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China.
Guohua MaGuangdong Provincial Key Laboratory of Applied Botany and Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China.
Kunlin WuGuangdong Provincial Key Laboratory of Applied Botany and Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China.
Lin FangGuangdong Provincial Key Laboratory of Applied Botany and Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China. linfang@scib.ac.cn.
Songjun ZengGuangdong Provincial Key Laboratory of Applied Botany and Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China. zengsongjun@scib.ac.cn.

Funding

Forestry Grassland Ecological Protection and Restoration Program E436070011Guangdong Basic and Applied Basic Research Foundation 2024A1515012952Guangdong Science and Technology Plan Project 2023B1212060046Guangzhou Science and Technology Program 2023E04J0004, 2024B03J1129Linzhi Science and Technology Program 2023-QYCX-02Science and Technology Projects in Guangzhou E33309
6 · The paper itself

Abstract

backgroundPaphiopedilum orchids have a high ornamental value, and flower abundance is a key horticultural trait. Most Paphiopedilum plants exhibit weak tillering ability, with their tiller buds often entering a dormant state post-formation. Tiller production plays a crucial role in enhancing flower abundance and is potentially regulated by plant hormones. However, the effect of hormones on tillering in Paphiopedilum plants is still unclear.

resultsIn this study, we investigated the promotion of tillering in P. callosum through exogenous root irrigation of benzylaminopurine (BAP). We observed a dose-dependent promotion of tiller production by BAP, with the strongest effect observed at a concentration of 400 mg/L. By comparing the expression of key genes in P. 'SCBG Yingchun' (with strong tiller ability) and P callosum (with weak tiller ability), we found that BAP promotes tillering by interacting with abscisic acid (ABA). This interaction involves down-regulation of the ABA degradation gene PcCYP707A, leading to a reduction in ABA content, and the subsequent down-regulation of dormancy-associated genes (PcDRMH1, PcSVP) to release bud dormancy. Additionally, BAP promotes sustained outgrowth of tiller buds by increasing the level of indole-3-acetic acid (IAA) through up-regulation of the IAA synthesis gene PcYUC2 and the transport gene PcPINIC.

conclusionsOur results indicated that the application of BAP promotes lateral bud outgrowth and increases tiller production in P. callosum. Through transcriptome analysis, we found that the BAP-promotion of tillering involves not only changes in endogenous IAA, ABA, and CTKs content but is also associated with the regulation of metabolism-related genes and dormancy-associated genes. This study presents the first comprehensive report of BAP-promoted tillering in P. callosum, providing a foundational basis for further mechanistic studies on tiller development in Paphiopedilum species and other non-model plants.

Indexed as

Benzyl CompoundsFlowersOrchidaceaePlant Growth RegulatorsPurinesAbscisic AcidGene Expression Regulation, PlantAbscisic AcidbenzylaminopurineBenzyl CompoundsPlant Growth RegulatorsPurinesCytokininGene expressionPaphiopedilumTillering regulationTranscriptome

Identifiers

PMID39988668
PMCPMC11849335

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.