Evidence map›Paper›PMID 39988434›Full record

ArticleEnvironmental microbiology2025

Co-Existence Slows Diversification in Experimental Populations of E. coli and P. fluorescens.

Gareth Howells, Aysha L Sezmis, Christopher Blake, Michael J McDonald

Abstract read
In one paragraph

Article in Environmental microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Gareth HowellsSchool of Biological Sciences, Monash University: Clayton, Clayton, Victoria, Australia.ORCID https://orcid.org/0009-0007-5946-8336
Aysha L SezmisSchool of Biological Sciences, Monash University: Clayton, Clayton, Victoria, Australia.
Christopher BlakeSchool of Biological Sciences, Monash University: Clayton, Clayton, Victoria, Australia.
Michael J McDonaldSchool of Biological Sciences, Monash University: Clayton, Clayton, Victoria, Australia.

Funding

Australian Research Council DP220103548National Health and Medical Research Council APP1186140
6 · The paper itself

Abstract

Microbes grown in heterogeneous laboratory environments can rapidly diversify into multiple, coexisting variants. While the genetic and evolutionary mechanisms of laboratory adaptive radiations are well studied, how the presence of other species alters the outcomes of diversification is less well understood. To test the effect of co-culture growth on the Pseudomonas fluorescens SBW25 adaptive radiation, Escherichia coli and P. fluorescens were cultured in monoculture and co-culture for 8 weeks. In P. fluorescens monoculture, Wrinkly and Smooth Spreader types rapidly evolved and were maintained over 8 weeks, while E. coli monocultures evolved two colony types, a big and a small colony variant. In contrast, we found that in co-culture, E. coli did not evolve small colony variants. Whole genome sequencing revealed the genetic basis of possible co-culture specific adaptations in both E. coli and P. fluorescens. Altogether, our data support that the presence of multiple species changed the outcome of adaptive radiation.

Indexed as

Escherichia coliPseudomonas fluorescensAdaptation, PhysiologicalCoculture TechniquesGenome, BacterialWhole Genome Sequencingcoexistenceexperimental evolutionmicrobial ecology

Identifiers

PMID39988434
PMCPMC11847636

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.