ArticleNucleic acids research2025
Disassembly of unstable RNA structures by an E. coli DEAD-box chaperone accelerates ribosome assembly.
Article in Nucleic acids research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Methyltransferase RsmB chaperonesScience advances · 2026Article
- Exchange dynamics and kinetic control of gene regulation complexes.Nature reviews. Molecular cell biology · 2026Review
- Elucidating structure-function relationships in the mammalian nucleolus.Nature reviews. Molecular cell biology · 2026Review
- Synthesis of Ultra-Large Fibrous Proteins from Bacteria via a Looped-Translation System.bioRxiv : the preprint server for biology · 2026Article
- Fibrillarin/Nop1 perturbs RNA folding and assembly independently of liquid-liquid phase separation.Nucleic acids research · 2026Article
- Nascent RNA Folding and RNP Assembly Revealed by Single-molecule Microscopy.Journal of molecular biology · 2026Review
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Authors and funding
2 authors.
Funding
Abstract
Ribosome synthesis in bacteria is coupled with transcription of the pre-ribosomal RNA (pre-rRNA), which must fold and assemble with 20 or more ribosomal proteins. In vitro, the Escherichia coli pre-16S rRNA misfolds during transcription, delaying stable binding of ribosomal protein uS4 that nucleates assembly of the 16S 5' domain. Using single-molecule fluorescence microscopy, we show that the DEAD-box protein CsdA (DeaD) strongly accelerates uS4 binding by facilitating proper folding of the nascent rRNA. Unstable RNA structures are unfolded by CsdA, whereas stable RNA structures resist unwinding. We show that CsdA unfolding becomes less frequent as more ribosomal proteins add to the complex. The results demonstrate that disassembly of unstable, nascent RNA-protein complexes by chaperones fuels the search for native structure. We propose that general chaperones create a gradient of disassembly that steepens the hierarchy of proper protein addition until late assembly intermediates escape unwinding and commit to 30S maturation.
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Registered trials
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