Evidence map›Paper›PMID 39985446›Full record

ArticleBioinformatics (Oxford, England)2025

SpectroPipeR-a streamlining post Spectronaut® DIA-MS data analysis R package.

Stephan Michalik, Elke Hammer, Leif Steil, Manuela Gesell Salazar, Christian Hentschker, Kristin Surmann, Larissa M Busch, Thomas Sura, Uwe Völker

Abstract read
In one paragraph

Article in Bioinformatics (Oxford, England), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Linking Virulence and Iron Limitation Response inJournal of proteome research · 2025
    Article
  6. Article
  7. Integrated genomic and proteomic analysis of the mouse-adaptedCurrent research in microbial sciences · 2025
    Article
  8. Article
  9. The global proteome ofFrontiers in cellular and infection microbiology · 2025
    Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Stephan MichalikDepartment Functional Genomics, Interfaculty Institute of Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, MV 17475, Germany.ORCID 0000-0002-6550-0132
Elke HammerDepartment Functional Genomics, Interfaculty Institute of Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, MV 17475, Germany.
Leif SteilDepartment Functional Genomics, Interfaculty Institute of Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, MV 17475, Germany.
Manuela Gesell SalazarDepartment Functional Genomics, Interfaculty Institute of Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, MV 17475, Germany.
Christian HentschkerDepartment Functional Genomics, Interfaculty Institute of Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, MV 17475, Germany.
Kristin SurmannDepartment Functional Genomics, Interfaculty Institute of Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, MV 17475, Germany.
Larissa M BuschDepartment Functional Genomics, Interfaculty Institute of Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, MV 17475, Germany.
Thomas SuraDepartment Functional Genomics, Interfaculty Institute of Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, MV 17475, Germany.
Uwe VölkerDepartment Functional Genomics, Interfaculty Institute of Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, MV 17475, Germany.ORCID 0000-0002-5689-3448

Funding

Computational Life Sciences
6 · The paper itself

Abstract

summaryProteome studies frequently encounter challenges in down-stream data analysis due to limited bioinformatics resources, rapid data generation, and variations in analytical methods. To address these issues, we developed SpectroPipeR, an R package designed to streamline data analysis tasks and provide a comprehensive, standardized pipeline for Spectronaut® DIA-MS data. This novel package automates various analytical processes, including XIC plots, ID rate summary, normalization, batch and covariate adjustment, relative protein quantification, multivariate analysis, and statistical analysis, while generating interactive HTML reports for e.g. ELN systems. AVAILABILITY AND IMPLEMENTATION: The SpectroPipeR package (manual: https://stemicha.github.io/SpectroPipeR/) was written in R and is freely available on GitHub (https://github.com/stemicha/SpectroPipeR).

Indexed as

Mass SpectrometryProteomeProteomicsSoftwareComputational BiologyProteome

Identifiers

PMID39985446
PMCPMC11893148

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.