Evidence map›Paper›PMID 39984626›Full record

ArticleScientific reports2025

Insights into metabolic changes during epidermal differentiation as revealed by multiphoton microscopy with fluorescence lifetime imaging.

Monika Malak, Chen Qian, Jeemol James, Syam Nair, Julie Grantham, Marica B Ericson

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PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Monika Malak *Department of Chemistry and Molecular Biology, Faculty of Science, University of Gothenburg, Gothenburg, 412 96, Sweden.
Chen Qian *Department of Chemistry and Molecular Biology, Faculty of Science, University of Gothenburg, Gothenburg, 412 96, Sweden. chen.qian@gu.se.
Jeemol JamesDepartment of Chemistry and Molecular Biology, Faculty of Science, University of Gothenburg, Gothenburg, 412 96, Sweden.
Syam NairInstitute of Neuroscience and Physiology, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, 413 90, Sweden.
Julie GranthamDepartment of Chemistry and Molecular Biology, Faculty of Science, University of Gothenburg, Gothenburg, 412 96, Sweden.
Marica B EricsonDepartment of Chemistry and Molecular Biology, Faculty of Science, University of Gothenburg, Gothenburg, 412 96, Sweden. marica.ericson@gu.se.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Rapid developments in the field of organotypic cultures have generated a growing need for effective and non-invasive methods for quality control during tissue development. In this study, we correlate metabolic changes with epidermal differentiation and demonstrate that multiphoton microscopy with fluorescence lifetime imaging (MPM-FLIM) can be applied to monitor epidermal differentiation of keratinocytes with respect to proliferative and differentiated states. In a 2D keratinocyte tissue culture model, increased expression of differentiation markers keratin-1 and keratin-10 was induced with calcium supplementation. An accompanying shift from glycolysis to mitochondrial respiration was detected in metabolic flux assays. Analysis of MPM-FLIM images acquired at 750 nm and 900 nm excitation revealed a decreased relative fraction of intracellular NADH and FAD after high calcium treatment, consistent with increased oxidative phosphorylation. Epidermal differentiation could be monitored over a 96 h period. Discrimination analysis based on k-means clustering generated clusters that correlated well with the duration of high Ca

Indexed as

Cell DifferentiationEpidermal CellsEpidermisKeratinocytesMicroscopy, Fluorescence, MultiphotonCalciumCells, CulturedHumansMitochondriaNADOptical ImagingOxidative PhosphorylationCalciumNADEpidermal differentiationFluorescence lifetime imagingLabel-free imagingMetabolismMultiphoton microscopy

Identifiers

PMID39984626
PMCPMC11845624

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.