ArticleBMC veterinary research2025
Analytical validation of an automated assay to measure calprotectin (S100A8/A9) in dog saliva and serum and changes in canine leishmaniasis, pyometra and hyperadrenocorticism.
Article in BMC veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Assessment of faecal calprotectin in neonatal calf diarrhoea using a human ELISA kit: Associations with faecal score and serum biochemical markers.Veterinary record open · 2026Article
- LeishVet update and current recommendations on feline leishmaniosis.Parasites & vectors · 2026Review
- Analytical validation of aldolase, microalbumin and myeloperoxidase in canine saliva and their evaluation in canine gingivitis.Scientific reports · 2026Article
- Transcriptome changes in overweight neutered female dogs undergoing a weight loss program with or without Spirulina (Arthrospira platensis) supplementation.BMC veterinary research · 2026Article
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5 authors.
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Abstract
backgroundCalprotectin (S100A8/A9) is a protein related to innate immunity that is considered a biomarker of inflammation. Currently, there is a commercially available automated assay for the measurement of calprotectin concentration (Bühlmann fCal Turbo
resultsThe analytical validation results showed that the assay was precise (coefficients of variation < 15% in all cases), accurate (the dilutional parallelism for serum and salivary calprotectin showed observed-to-expected ratios with a mean of 96.9% and 97.2%, respectively), and presented a limit of detection of 0.038 mg/L. When this assay was applied to the different diseases, a significant increase in the concentration of salivary calprotectin in dogs with leishmaniasis (p = 0.0002) and in those with pyometra (p = 0.002), compared to healthy ones, was observed, whereas no significant differences were found in serum. Furthermore, a significant positive moderate correlation was obtained between salivary calprotectin and serum CRP (r = 0.5; p = 0.001) and haptoglobin (r = 0.5; p = 0.002), and between calprotectin and CRP (r = 0.67; p < 0.001) in serum.
conclusionsCalprotectin (S100A8/A9) can be measured in dog saliva and serum samples by the automated method validated in this study, and when measured in saliva it could be used as a potential biomarker of inflammation and immune activation in the dog.
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