Evidence map›Paper›PMID 39979987›Full record

ArticleMalaria journal2025

Anopheles arabiensis larval habitats characterization and Anopheles species diversity in water bodies from Jozini, KwaZulu-Natal Province.

Elliot Marubini, Alfred Musekiwa, Innocent Maposa, Theresa Mazarire, Windy Sekgele, Nondumiso Mabaso, Dumsani Dlamini, Hetani Mdose, Lazarus Kuonza, Givemore Munhenga

Abstract read
In one paragraph

Article in Malaria journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Elliot MarubiniSouth African Field Epidemiology Training Programme, National Institute for Communicable Diseases, Johannesburg, South Africa. elliot.marubini@gmail.com.
Alfred MusekiwaSchool of Health Systems and Public Health, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.
Innocent MaposaDivision of Epidemiology and Biostatistics, Department of Global Health, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa.
Theresa MazarireVector Reference Laboratory, Centre for Emerging Zoonotic and Parasitic Diseases, National Institute for Communicable Diseases, National Health Laboratory Services, Johannesburg, South Africa.
Windy SekgeleVector Reference Laboratory, Centre for Emerging Zoonotic and Parasitic Diseases, National Institute for Communicable Diseases, National Health Laboratory Services, Johannesburg, South Africa.
Nondumiso MabasoWits Research Institute for Malaria, Faculty of Health Sciences, University of the Witwatersrand, 7 York Road, Parktown, Johannesburg, 2193, South Africa.
Dumsani DlaminiWits Research Institute for Malaria, Faculty of Health Sciences, University of the Witwatersrand, 7 York Road, Parktown, Johannesburg, 2193, South Africa.
Hetani MdoseSouth African Field Epidemiology Training Programme, National Institute for Communicable Diseases, Johannesburg, South Africa.
Lazarus KuonzaSouth African Field Epidemiology Training Programme, National Institute for Communicable Diseases, Johannesburg, South Africa.
Givemore MunhengaVector Reference Laboratory, Centre for Emerging Zoonotic and Parasitic Diseases, National Institute for Communicable Diseases, National Health Laboratory Services, Johannesburg, South Africa.

Funding

Department of Science and Innovation Health Scheme Awarded to Dr GivemoreInternational Atomic Energy Agency Technical Cooperation Programme SAF 5017, research contract 19099National Health Laboratory Service Awarded to Dr GivemoreResearch Trust and Technology Agency Awarded to Dr GivemoreSouth African Field Epidemiology Training Programme ScholarshipSouth African National Research Foundation 107428South African National Research Foundation 119765
6 · The paper itself

Abstract

backgroundThe South African government is now implementing winter larviciding as a supplementary vector control tool. To achieve effective larviciding programme there is a need to understand the distribution of the breeding sites of vectors and their corresponding ecology. This study aimed to determine larval breeding sites of anophelines and characterize the physicochemical properties of water that promote the proliferation of Anopheles arabiensis immature stages.

methodsA desktop survey of water bodies was carried out followed by a physical search of potential Anopheles breeding sites. Anopheline larvae were sampled from breeding sites in January and April 2021. At each breeding site, physicochemical characteristics of the water, including pH, electrical conductivity, total dissolved solids, salinity and turbidity, were measured. The collected Anopheles larvae were reared to adults and identified to genus and species level using morphological and molecular techniques. Factors associated with the presence of An. arabiensis larvae in the breeding sites were determined.

resultsOut of the 72 water bodies identified using desktop survey only 53% (n = 38/72) were identified through physical search. Of these 84% (n = 32/38) were positive for Anopheles larvae. A total of 598 Anopheles larvae were collected, of which 59.4% (n = 355/598) emerged into adults. Morphological identification of these adults, showed that the Anopheles gambiae complex accounted for 70% (n = 250/355) of the collections. From the 250 An. gambiae complex collected, 94% (235/250) were identified to species level by PCR and 6% (n = 15/250) failed to amplify. Of the 235 An. gambiae complex that were identified to species level, 62.5% (n = 147/235) were from January collections and 37.4% (n = 88/235) were from April collections. Molecular identification of the An. gambiae complex to species level showed predominance of An. arabiensis in April, 91% (n = 80/88). All physicochemical parameters differed significantly between the breeding site classes (p < 0.05 in all instances), except for electrical conductivity (p = 0.07). The aquatic habitats surveyed showed that the impermanency of the water bodies, neutral to alkaline pH, moderate salinity and low total dissolved solids were associated with the occurrence of An. arabiensis larvae.

conclusionThis study showed that An. arabiensis primarily breed in small temporary water bodies characterized by neutral pH.

Indexed as

AnophelesBiodiversityEcosystemMosquito VectorsAnimalsLarvaSouth AfricaAnophelesLarvicidingMalariaVector

Identifiers

PMID39979987
PMCPMC11844105

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.