Evidence map›Paper›PMID 39975910›Full record

ArticleResearch square2025

Human Organ Chips Reveal New Inflammatory Bowel Disease Drivers.

Alican Özkan, Gwenn E Merry, David B Chou, Ryan R Posey, Anna Stejskalova, Karina Calderon, Megan Sperry, Viktor Horvath, Lorenzo E Ferri, Emanuela Carlotti and 7 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in Research square, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

17 authors.

Alican ÖzkanWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA.
Gwenn E MerryWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA.
David B ChouWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA.
Ryan R PoseyWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA.
Anna StejskalovaWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA.ORCID 0000-0003-4813-6452
Karina CalderonWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA.
Megan SperryWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA.
Viktor HorvathWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA.
Lorenzo E FerriThoracic and Upper GI Cancer Research Laboratories, Research Institute of McGill University Health Centre, Montreal, QC, Canada.
Emanuela CarlottiClonal Dynamics in Epithelia Laboratory, Queen Mary University of London, London, United Kingdom.
Stuart A C McDonaldClonal Dynamics in Epithelia Laboratory, Queen Mary University of London, London, United Kingdom.
Douglas J WintonCancer Research UK Cambridge Institute, Li Ka Shing Centre, Cambridge, United Kingdom.
Rocco RiccardiDepartment of Surgery, Massachusetts General Hospital, Boston, MA.
Liliana BordeianouDepartment of Surgery, Massachusetts General Hospital, Boston, MA.
Sean HallWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA.
Girija GoyalWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA.
Donald E IngberWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA.ORCID 0000-0002-4319-6520

Funding

RESEARCH TRAINING IN NEPHROLOGYT32DK007199 · NIDDK · BETH ISRAEL DEACONESS MEDICAL CENTER · PI POLLAK, MARTIN R. · 1986 to 2022
$6.0M
Organ Design and Engineering Training Program (ODET Program)T32EB016652 · NIBIB · BRIGHAM AND WOMEN'S HOSPITAL · PI BONVENTRE, JOSEPH VINCENT · 2014 to 2023
$3.5M
NIBIB NIH HHS T32 EB016652NIDDK NIH HHS T32 DK007199
6 · The paper itself

Abstract

Inflammatory bowel disease (IBD) patients exhibit compromised intestinal barrier function and decreased mucus accumulation, as well as increased inflammation, fibrosis, and cancer risk, with symptoms often being exacerbated in women during pregnancy. Here, we show that these IBD hallmarks can be replicated using human Organ Chips lined by IBD patient-derived colon epithelial cells interfaced with matched fibroblasts cultured under flow. Use of heterotypic tissue recombinants revealed that IBD fibroblasts are the primary drivers of multiple IBD symptoms. Inflammation and fibrosis are accentuated by peristalsis-like motions in IBD Chips and when exposed to pregnancy-associated hormones in female IBD Chips. Carcinogen exposure also increases inflammation, gene mutations, and chromosome duplication in IBD Chips, but not in Healthy Chips. These data enabled by human Organ Chip technology suggest that the intestinal stroma, sex hormones, and peristalsis-associated mechanical deformations play a key role in driving inflammation, fibrosis, and disease progression in male and female IBD patients.

Indexed as

CarcinogenesisCrohn’s DiseaseEpithelial-Stromal InteractionsOrgan-on-a-chipUlcerative ColitisWomen’s Health

Identifiers

PMID39975910
PMCPMC11838723

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.