Evidence map›Paper›PMID 39975479›Full record

ArticleFrontiers in parasitology2024

Application of a new highly multiplexed amplicon sequencing tool to evaluate

Jacob M Sadler, Alfred Simkin, Valery P K Tchuenkam, Isabela Gerdes Gyuricza, Abebe A Fola, Kevin Wamae, Ashenafi Assefa, Karamoko Niaré, Kyaw Thwai, Samuel J White and 8 more

Abstract read
In one paragraph

Article in Frontiers in parasitology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Trial
  2. Article
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  5. Complexity of Infection andmedRxiv : the preprint server for health sciences · 2026
    Article
  6. Haplotype diversity ofFrontiers in parasitology · 2026
    Article
  7. Article
  8. Article
  9. Article
  10. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

18 authors.

Jacob M SadlerInstitute for Global Health and Infectious Diseases, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States.
Alfred SimkinDepartment of Pathology and Laboratory Medicine, Warren Alpert Medical School, Brown University, Providence, RI, United States.
Valery P K TchuenkamDepartment of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon.
Isabela Gerdes GyuriczaInstitute for Global Health and Infectious Diseases, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States.
Abebe A FolaDepartment of Pathology and Laboratory Medicine, Warren Alpert Medical School, Brown University, Providence, RI, United States.
Kevin WamaeKenyan Medical Research Institute (KEMRI)-Wellcome Trust Research Programme, Kilifi, Kenya.
Ashenafi AssefaInstitute for Global Health and Infectious Diseases, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States.
Karamoko NiaréDepartment of Pathology and Laboratory Medicine, Warren Alpert Medical School, Brown University, Providence, RI, United States.
Kyaw ThwaiInstitute for Global Health and Infectious Diseases, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States.
Samuel J WhiteInstitute for Global Health and Infectious Diseases, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States.
William J MossDepartment of Epidemiology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, United States.
Rhoel R DinglasanDepartment of Infectious Diseases & Immunology, College of Veterinary Medicine & Emerging Pathogens Institute, University of Florida, Gainesville, FL, United States.
Sandrine Eveline NsangoMalaria Research Unit, Centre Pasteur du Cameroun, Yaoundé, Cameroon.
Christopher B TumeDepartment of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon.
Jonathan B ParrInstitute for Global Health and Infectious Diseases, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States.
Innocent Mbulli AliDepartment of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon.
Jeffrey A BaileyDepartment of Pathology and Laboratory Medicine, Warren Alpert Medical School, Brown University, Providence, RI, United States.
Jonathan J JulianoInstitute for Global Health and Infectious Diseases, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States.

Funding

TransmissionU19AI089680 · NIAID · JOHNS HOPKINS UNIVERSITY · PI WILLIAM J MOSS · 2010 to 2026
$25.6M
Relapsing malaria in Africa: mechanisms for persistence amid falciparum declineR01AI165537 · NIAID · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Rhoel David Ramos Dinglasan, Jonathan J Juliano · 2022 to 2026
$3.6M
Epidemiology and determinants of emerging artemisinin-resistant malaria in EthiopiaR01AI177791 · NIAID · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Jonathan Boyd Parr · 2023 to 2026
$3.2M
West-Central Africa EMERGENTS International Center of Excellence in Malaria Research (ICEMR)U19AI181594 · NIAID · UNIVERSITY OF FLORIDA · PI Rhoel David Ramos Dinglasan · 2024 to 2026
$3.0M
Artemisinin Resistance in Africa: its emergence and evolution in RwandaR01AI156267 · NIAID · BROWN UNIVERSITY · PI BAILEY, JEFFREY A., JULIANO, JONATHAN J · 2021 to 2024
$3.0M
Importation and transmission of malaria in Zanzibar: a case study for eliminationR01AI155730 · NIAID · UNIV OF NORTH CAROLINA CHAPEL HILL · PI JULIANO, JONATHAN J, LIN, JESSICA · 2021 to 2024
$2.8M
Mentoring in Translational Malaria GenomicsK24AI134990 · NIAID · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Jonathan J Juliano · 2018 to 2026
$1.6M
NIAID NIH HHS K24 AI134990NIAID NIH HHS R01 AI155730NIAID NIH HHS R01 AI156267NIAID NIH HHS R01 AI165537NIAID NIH HHS R01 AI177791NIAID NIH HHS U19 AI089680NIAID NIH HHS U19 AI181594
6 · The paper itself

Abstract

Background: Resistance to antimalarial drugs remains a major obstacle to malaria elimination. Multiplexed, targeted amplicon sequencing is being adopted for surveilling resistance and dissecting the genetics of complex malaria infections. Moreover, genotyping of parasites and detection of molecular markers drug resistance in resource-limited regions requires open-source protocols for processing samples, using accessible reagents, and rapid methods for processing numerous samples including pooled sequencing. Methods: Results: We evaluated Conclusion: Overall,

Indexed as

amplicon sequencingantimalarial resistancegenetic relatednessmalariaPlasmodium falciparum

Identifiers

PMID39975479
PMCPMC11835963

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.