Evidence map›Paper›PMID 39975286›Full record

ArticlebioRxiv : the preprint server for biology2025

A Sensitive Soma-localized Red Fluorescent Calcium Indicator for Multi-Modality Imaging of Neuronal Populations In Vivo.

Shihao Zhou, Qiyu Zhu, Minho Eom, Shilin Fang, Oksana M Subach, Chen Ran, Jonnathan Singh Alvarado, Praneel S Sunkavalli, Yuanping Dong, Yangdong Wang and 10 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

20 authors.

Shihao ZhouSchool of Life Sciences, Westlake University, Hangzhou, Zhejiang, China.
Qiyu ZhuIDG/McGovern Institute for Brain Research, Tsinghua University, Beijing 100084, China.
Minho EomSchool of Electrical Engineering, KAIST, Daejeon, Republic of Korea.
Shilin FangInstitute of Neuroscience, State Key Laboratory of Neuroscience, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China.
Oksana M SubachComplex of NBICS Technologies, National Research Center "Kurchatov Institute", Moscow, 123182, Russia.
Chen RanDepartment of Neuroscience, Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, CA 92037, USA.
Jonnathan Singh AlvaradoDivision of Endocrinology, Diabetes and Metabolism, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
Praneel S SunkavalliDivision of Endocrinology, Diabetes and Metabolism, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
Yuanping DongSchool of Life Sciences, Westlake University, Hangzhou, Zhejiang, China.
Yangdong WangSchool of Life Sciences, Westlake University, Hangzhou, Zhejiang, China.
Jiewen HuInstitute of Neuroscience, State Key Laboratory of Neuroscience, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China.
Hanbin ZhangSchool of Life Sciences, Westlake University, Hangzhou, Zhejiang, China.
Zhiyuan WangInstitute of Neuroscience, State Key Laboratory of Neuroscience, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China.
Xiaoting SunSchool of Life Sciences, Westlake University, Hangzhou, Zhejiang, China.
Tao YangSchool of Life Sciences, Westlake University, Hangzhou, Zhejiang, China.
Yu MuInstitute of Neuroscience, State Key Laboratory of Neuroscience, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China.ORCID 0000-0001-9297-4854
Young-Gyu YoonSchool of Electrical Engineering, KAIST, Daejeon, Republic of Korea.
Zengcai V GuoIDG/McGovern Institute for Brain Research, Tsinghua University, Beijing 100084, China.
Fedor V SubachComplex of NBICS Technologies, National Research Center "Kurchatov Institute", Moscow, 123182, Russia.
Kiryl D PiatkevichSchool of Life Sciences, Westlake University, Hangzhou, Zhejiang, China.ORCID 0000-0002-7777-9468

Funding

Nikon A1-R multi-photon microscope for a core microscopy facilityS10OD026817 · OD · SCRIPPS RESEARCH INSTITUTE, THE · PI HENDERSON, SCOTT C. · 2019 to 2019
$837k
Deconstructing brainstem circuits for visceral sensesK01DK132957 · NIDDK · SCRIPPS RESEARCH INSTITUTE, THE · PI Chen Ran · 2023 to 2026
$586k
NIDDK NIH HHS K01 DK132957NIH HHS S10 OD026817
6 · The paper itself

Abstract

Recent advancements in genetically encoded calcium indicators, particularly those based on green fluorescent proteins, have optimized their performance for monitoring neuronal activities in a variety of model organisms. However, progress in developing red-shifted GECIs, despite their advantages over green indicators, has been slower, resulting in fewer options for end-users. In this study, we explored topological inversion and soma-targeting strategies, which are complementary to conventional mutagenesis, to re-engineer a red genetically encoded calcium indicator, FRCaMP, for enhanced

Indexed as

FRCaMPigenetically encoded calcium indicatorneuropil contaminationone-photon wide-field calcium imagingprotein engineeringred fluorescent proteinSchizosaccharomyces pombetwo-photon calcium imaging

Identifiers

PMID39975286
PMCPMC11838422

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.