Evidence map›Paper›PMID 39975277›Full record

ArticlebioRxiv : the preprint server for biology2025

Next-Generation Protein Sequencing and individual ion mass spectrometry enable complementary analysis of interleukin-6.

Kenneth A Skinner, Troy D Fisher, Andrew Lee, Taojunfeng Su, Eleonora Forte, Aniel Sanchez, Michael A Caldwell, Neil L Kelleher

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Kenneth A SkinnerQuantum-Si, Incorporated, Branford, Connecticut, United States.ORCID 0000-0001-6407-5034
Troy D FisherProteomics Center of Excellence, Northwestern University, Evanston, Illinois, United States.ORCID 0009-0006-1306-0397
Andrew LeeDepartments of Molecular Biosciences, Chemistry and Chemical and Biological Engineering, Northwestern University, Evanston, IL, United States.ORCID 0000-0002-7665-1999
Taojunfeng SuDepartments of Molecular Biosciences, Chemistry and Chemical and Biological Engineering, Northwestern University, Evanston, IL, United States.ORCID 0000-0002-7111-1248
Eleonora ForteProteomics Center of Excellence, Northwestern University, Evanston, Illinois, United States.ORCID 0000-0002-6095-2797
Aniel SanchezProteomics Center of Excellence, Northwestern University, Evanston, Illinois, United States.ORCID 0000-0002-0278-8802
Michael A CaldwellProteomics Center of Excellence, Northwestern University, Evanston, Illinois, United States.ORCID 0000-0002-8636-0706
Neil L KelleherProteomics Center of Excellence, Northwestern University, Evanston, Illinois, United States.ORCID 0000-0002-8815-3372

Funding

TR&D 7: Cell Specific ProteomicsP41GM108569 · NIGMS · NORTHWESTERN UNIVERSITY · PI KELLEHER, NEIL L · 2015 to 2024
$13.6M
NIGMS NIH HHS P41 GM108569
6 · The paper itself

Abstract

The vast complexity of the proteome currently overwhelms any single analytical technology in capturing the full spectrum of proteoform diversity. In this study, we evaluated the complementarity of two cutting-edge proteomic technologies-single-molecule protein sequencing and individual ion mass spectrometry-for analyzing recombinant human IL-6 (rhIL-6) at the amino acid, peptide, and intact proteoform levels. For single-molecule protein sequencing, we employ the recently released Platinum

Indexed as

cytokineindividual ion mass spectrometryNext-Generation Protein SequencingPlatinumproteoformSingle-molecule protein sequencingtop-down mass spectrometry

Identifiers

PMID39975277
PMCPMC11839055

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.