Evidence map›Paper›PMID 39974948›Full record

ArticlebioRxiv : the preprint server for biology2025

Thermodynamics shape the

Daven B Khana, Annie Jen, Evgenia Shishkova, Eashant Thusoo, Jonathan Williams, Alex Henkel, David M Stevenson, Joshua J Coon, Daniel Amador-Noguez

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Daven B KhanaDepartment of Bacteriology, University of Wisconsin-Madison, Madison WI USA.ORCID 0000-0002-8081-3436
Annie JenDepartment of Biomolecular Chemistry, University of Wisconsin-Madison, Madison WI USA.ORCID 0000-0001-5569-8387
Evgenia ShishkovaDepartment of Biomolecular Chemistry, University of Wisconsin-Madison, Madison WI USA.ORCID 0000-0001-6097-6097
Eashant ThusooDepartment of Bacteriology, University of Wisconsin-Madison, Madison WI USA.
Jonathan WilliamsDepartment of Bacteriology, University of Wisconsin-Madison, Madison WI USA.
Alex HenkelUniversity of Wisconsin-Madison Carbone Cancer Center, University of Wisconsin-Madison, Madison WI USA.ORCID 0009-0003-0608-7581
David M StevensonDepartment of Bacteriology, University of Wisconsin-Madison, Madison WI USA.
Joshua J CoonGreat Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison WI USA.
Daniel Amador-NoguezCenter for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA.ORCID 0000-0002-3568-3070

Funding

TR&D 2 Metabolic Labels for Ultraplexed Protein Quantification p. 453P41GM108538 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI COON, JOSHUA J · 2016 to 2025
$13.1M
Structure, Function and Regulation of the ProteomeR35GM118110 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI COON, JOSHUA J · 2016 to 2025
$9.1M
NIGMS NIH HHS P41 GM108538NIGMS NIH HHS R35 GM118110
6 · The paper itself

Abstract

Thermodynamically constrained reactions and pathways are hypothesized to impose greater protein demands on cells, requiring higher enzyme amounts to sustain a given flux compared to those with stronger thermodynamics. To test this, we quantified the absolute concentrations of glycolytic enzymes in three bacterial species -

Identifiers

PMID39974948
PMCPMC11838459

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.