Evidence map›Paper›PMID 39974898›Full record

ArticlebioRxiv : the preprint server for biology2025

Structural basis of undecaprenyl phosphate glycosylation leading to polymyxin resistance in Gram-negative bacteria.

Khuram U Ashraf, Mariana Bunoro-Batista, T Bertie Ansell, Ankita Punetha, Stephannie Rosario-Garrido, Emre Firlar, Jason T Kaelber, Phillip J Stansfeld, Vasileios I Petrou

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Khuram U AshrafDepartment of Microbiology, Biochemistry, and Molecular Genetics, New Jersey Medical School, Rutgers Biomedical Health Sciences, Newark, NJ, 07103, USA.ORCID 0000-0002-0799-4947
Mariana Bunoro-BatistaSchool of Life Sciences and Department of Chemistry, University of Warwick, Coventry, United Kingdom.ORCID 0000-0002-2468-748X
T Bertie AnsellDepartment of Biochemistry, University of Oxford, Oxford, United Kingdom.ORCID 0000-0003-4870-5387
Ankita PunethaDepartment of Microbiology, Biochemistry, and Molecular Genetics, New Jersey Medical School, Rutgers Biomedical Health Sciences, Newark, NJ, 07103, USA.ORCID 0000-0002-2845-5459
Stephannie Rosario-GarridoDepartment of Microbiology, Biochemistry, and Molecular Genetics, New Jersey Medical School, Rutgers Biomedical Health Sciences, Newark, NJ, 07103, USA.ORCID 0009-0006-3350-6448
Emre FirlarRutgers Cryo-EM & Nanoimaging Facility, Rutgers, the State University of New Jersey, Piscataway, NJ 08854, USA.ORCID 0000-0003-0190-6528
Jason T KaelberRutgers Cryo-EM & Nanoimaging Facility, Rutgers, the State University of New Jersey, Piscataway, NJ 08854, USA.ORCID 0000-0001-9426-1030
Phillip J StansfeldSchool of Life Sciences and Department of Chemistry, University of Warwick, Coventry, United Kingdom.ORCID 0000-0001-8800-7669
Vasileios I PetrouDepartment of Microbiology, Biochemistry, and Molecular Genetics, New Jersey Medical School, Rutgers Biomedical Health Sciences, Newark, NJ, 07103, USA.ORCID 0000-0002-3194-9863

Funding

NCCAT: National Center for CryoEM Access and Training- Supplement for Windows 10 and FFIU24GM129539 · NIGMS · NEW YORK STRUCTURAL BIOLOGY CENTER · PI DE MARCO, ALEX, KIEFT, JEFFREY S · 2018 to 2023
$53.9M
The role of cardiolipin in the biogenesis of the Gram-negative bacterial cell envelopeR01AI174416 · NIAID · UNIVERSITY OF GEORGIA · PI Michael Stephen Trent · 2023 to 2026
$2.4M
Structure and mechanism of membrane enzymes responsible for bacterial lipid modification and polymyxin resistanceR35GM150831 · NIGMS · RUTGERS BIOMEDICAL AND HEALTH SCIENCES · PI Vasileios I Petrou · 2023 to 2026
$1.6M
STRUCTURAL BASIS OF AMINORABINOSE BIOSYNTHESIS LINKED TO POLYMYXIN RESISTANCER00GM123228 · NIGMS · RBHS-NEW JERSEY MEDICAL SCHOOL · PI PETROU, VASILEIOS I · 2019 to 2021
$747k
NIAID NIH HHS R01 AI174416NIGMS NIH HHS R00 GM123228NIGMS NIH HHS R35 GM150831NIGMS NIH HHS U24 GM129539Wellcome Trust
6 · The paper itself

Abstract

In Gram-negative bacteria, the enzymatic modification of Lipid A with aminoarabinose (L-Ara4N) leads to resistance against polymyxin antibiotics and cationic antimicrobial peptides. ArnC, an integral membrane glycosyltransferase, attaches a formylated form of aminoarabinose to the lipid undecaprenyl phosphate, enabling its association with the bacterial inner membrane. Here, we present cryo-electron microscopy structures of ArnC from

Indexed as

aminoarabinoseglycosyltransferaselipopolysaccharidemembrane proteinPolymyxin resistancePren-P GTundecaprenyl phosphate

Identifiers

PMID39974898
PMCPMC11838356

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.