ArticleJournal of animal science and technology2025
Comparative miRNome analysis of colostrum- and mature milk-derived extracellular vesicles from Holstein and Jersey cows.
Article in Journal of animal science and technology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The trial behind it
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Who cites it
8 citing papers in PubMed.
- Investigating Dietary microRNA Stability and Function Using a Transgenic Milk Model With Unique microRNA Sequences.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- Cross-Species Analysis of Milk Extracellular Vesicles Reveals a Conserved Innate Core and a Divergent, Human-Specific Adaptive Immune Fraction.International journal of molecular sciences · 2026Article
- Bacterial Extracellular Vesicles as Regulators of the Gut Microbiome-Circadian Axis in Inflammatory and Metabolic Diseases.Journal of microbiology and biotechnology · 2026Review
- MicroRNAs from colostrum and mature cow milk: High-throughput sequencing and functional enrichment.Food chemistry. Molecular sciences · 2026Article
- Comprehensive Analysis of Exosomal microRNAs in Buffalo Milk Across the Early Postpartum Transition.Molecules (Basel, Switzerland) · 2026Article
- Predominant miRNAs in Animal-Source Foods and Bioinformatic Analysis.Current issues in molecular biology · 2026Article
- Differential protein abundance in colostrum-derived extracellular vesicles from bovine leukemia virus-infected cattle are implicated in immune regulation and inflammation.Frontiers in immunology · 2026Article
- Milk-Derived Extracellular Vesicles and microRNAs: Potential Modulators of Intestinal Homeostasis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs (miRNAs) are small noncoding RNAs that play a pivotal role in the regulation of gene expression. Analysis of miRNAs is important for understanding a variety of biological processes. Sequencing of miRNAs within milk-derived extracellular vesicles (EVs) provides valuable insights into the molecular mechanisms through which these EVs influence recipient cells. Comparative miRNA sequencing of colostrum and mature milk from different cow breeds can demonstrate breed-specific differences and improve the understanding of potential therapeutic applications in immune regulation and gut health. Therefore, this study was conducted to compare the miRNA profiles and characteristics of colostrum- and mature milk-derived EVs from Holstein and Jersey breeds and determine their effects on intestinal epithelial cells. The miRNA profiles of EVs isolated from the colostrum and mature milk of Holstein and Jersey cows were analyzed via small RNA sequencing. Holstein colostrum-derived EVs exhibited the most diverse miRNA profile with 421 identified miRNAs compared with 259 in mature milk-derived EVs. Jersey colostrum EVs had 198 miRNAs, whereas mature milk EVs had 282. Differential expression analysis revealed considerable miRNA differences between colostrum and mature milk, particularly in Holstein cows. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses revealed that miRNAs from colostrum EVs predominantly regulated immune-related pathways. Transcriptomic analysis of human colon cell line HT-29 treated with Holstein colostrum EVs confirmed the modulation of genes associated with immune responses. These findings indicate that colostrum-derived EVs, particularly from Holstein cows, play a pivotal role in immune regulation and could be potential candidates for therapeutic applications.
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