Evidence map›Paper›PMID 39974521›Full record

ArticleFood chemistry: X2025

Development of a method for simultaneous analysis of glycosaminoglycan disaccharides and evaluating the quality of chondroitin sulfate and hyaluronic acid in food raw materials.

Kengo Hirai, Takamasa Ishii, Ayaka Aijima, Nae Yokota, Yasuhisa Miyamoto, Kyohei Higashi, Yusuke Iwasaki, Rie Ito, Nobuaki Higashi, Hiroshi Akiyama

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Food chemistry: X, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Kengo HiraiAnalytical Science Laboratories, Asahi Quality & Innovations, Ltd., 1-21 Midori 1-Chome, Moriya, Ibaraki 302-0106, Japan.
Takamasa IshiiDepartment of Analytical Chemistry, School of Pharmacy and Pharmaceutical Sciences, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.
Ayaka AijimaDepartment of Analytical Chemistry, School of Pharmacy and Pharmaceutical Sciences, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.
Nae YokotaDepartment of Analytical Chemistry, School of Pharmacy and Pharmaceutical Sciences, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.
Yasuhisa MiyamotoAnalytical Science Laboratories, Asahi Quality & Innovations, Ltd., 1-21 Midori 1-Chome, Moriya, Ibaraki 302-0106, Japan.
Kyohei HigashiFaculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan.
Yusuke IwasakiDepartment of Analytical Chemistry, School of Pharmacy and Pharmaceutical Sciences, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.
Rie ItoDepartment of Analytical Chemistry, School of Pharmacy and Pharmaceutical Sciences, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.
Nobuaki HigashiDepartment of Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.
Hiroshi AkiyamaDepartment of Analytical Chemistry, School of Pharmacy and Pharmaceutical Sciences, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We developed a comprehensive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the simultaneous analysis of 19 glycosaminoglycan disaccharide components. Although structural similarity among components can hinder MS identification, we optimized the LC conditions to successfully separate all components. Specifically, we quantitatively and qualitatively evaluated chondroitin sulfate (CS) and hyaluronic acid (HA) in food ingredients. The method involves enzymatic degradation of CS and HA by Chondroitinase ABC into their constituent disaccharides, followed by LC-MS/MS quantification. We identified conditions that ensure optimal enzymatic digestion of CS and HA. Calibration curves showed excellent precision, accuracy, and linearity in validation tests. Quantitative analyses of CS and HA in commercially available raw materials were consistent with labeled purity. Disaccharide composition analysis suggested that the origin stated on the label was accurate. The findings suggest that the proposed method is useful for the quantitative and qualitative evaluation of the functional food ingredients CS and HA.

Indexed as

Chondroitin sulfateEnzymatic digestionGlycosaminoglycanHyaluronic acidLC-MS/MSUnsaturated disaccharide

Identifiers

PMID39974521
PMCPMC11838141

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.