ArticleFrontiers in veterinary science2025
Establishment of an indirect ELISA method for detecting bovine coronavirus antibodies based on N protein.
Article in Frontiers in veterinary science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Molecular epidemiology of group A rotavirus (RVA) and bovine coronavirus (BCoV) associated with neonatal calf diarrhea in Türkiye.Tropical animal health and production · 2026Article
- Diagnostic Methods for Bovine Coronavirus: A Review of Recent Advancements and Challenges.Viruses · 2025Review
- Development of Immunodetection Systems Using a Specific Antibody Against the Recombinant Coat Protein for Detecting Sugarcane Streak Mosaic Virus.Pathogens (Basel, Switzerland) · 2025Article
- Multiplex one-step RT-qPCR assays for simultaneous detection of BRV, BCoV,Frontiers in veterinary science · 2025Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bovine Coronavirus (BCoV) is a significant pathogen responsible for neonatal calf diarrhea, winter dysentery in adult cattle, and bovine respiratory diseases. Infection with the virus can result in hemorrhagic diarrhea, decreased milk production, and potentially fatal outcomes in cattle, leading to considerable economic repercussions for the cattle industry. Efficient management of BCoV relies on swift and precise detection techniques. CHO cells were utilized to express a secreted recombinant nucleocapsid protein (N), whereby rabbit polyclonal antibodies (pAb) were generated through immunization. An indirect enzyme-linked immunosorbent assay (iELISA) based on N protein was established for the detection of BCoV antibodies. Reaction conditions were optimized using a checkerboard approach, with the optimal antigen concentration at 1.25 μg/mL and the optimal antibody dilution at 1:200, the cutoff value distinguishing negative and positive serum samples was 0.986. The sensitivity test indicated that this rabbit pAb had a maximum dilution of 2
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