Evidence map›Paper›PMID 39972250›Full record

ArticleBMC plant biology2025

GhADT5 enhances alkali stress tolerance in cotton by regulating phenylalanine-derived flavonoid biosynthesis and antioxidant defense.

Lidong Wang, Hongyu Nan, Menghao Zhang, Lijun Guang, Junting Meng, Mengyue Liu, Yuan Meng, Wenhua Chen, Yapeng Fan, Hui Huang and 16 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

26 authors.

Lidong Wang *State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Anyang, Henan, 455000, China.
Hongyu Nan *Institute of Crop Sciences, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu, 730070, China.
Menghao Zhang *State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Anyang, Henan, 455000, China.
Lijun Guang *Institute of Agricultural Science of 13th Division of Xinjiang Production and Construction Corps, Hami, Xinjiang, 839000, China.
Junting Meng *State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Anyang, Henan, 455000, China.
Mengyue LiuState Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Anyang, Henan, 455000, China.
Yuan MengState Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Anyang, Henan, 455000, China.
Wenhua ChenState Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Anyang, Henan, 455000, China.
Yapeng FanState Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Anyang, Henan, 455000, China.
Hui HuangState Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Anyang, Henan, 455000, China.
Yuping SunState Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Anyang, Henan, 455000, China.
Zhining YangState Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Anyang, Henan, 455000, China.
Xiao ChenState Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Anyang, Henan, 455000, China.
Fange WuState Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Anyang, Henan, 455000, China.
Ruize SongState Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Anyang, Henan, 455000, China.
Shuai WangState Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Anyang, Henan, 455000, China.
Xuke LuState Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Anyang, Henan, 455000, China.
Xiugui ChenState Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Anyang, Henan, 455000, China.
Lanjie ZhaoState Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Anyang, Henan, 455000, China.
Junjuan WangState Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Anyang, Henan, 455000, China.
Yupeng CuiState Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Anyang, Henan, 455000, China.
Xue-Rong ZhouCSIRO Agriculture and Food, PO Box 1700, Canberra, ACT, 2601, Australia.
Ning WangInstitute of Crop Sciences, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu, 730070, China.
Keyun FengInstitute of Crop Sciences, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu, 730070, China.
Qin ChenEngineering Research Centre of Cotton, Ministry of Education / College of Agriculture, Xinjiang Agricultural University, 311 Nongda East Road, Urumqi, Xinjiang, 830052, China. cqq0707@sina.com.
Wuwei YeState Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Anyang, Henan, 455000, China. yew158@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Phenylalanine (Phe), an aromatic amino acid, is a key precursor of flavonoids, which are crucial for plant growth and development. Arogenate dehydratase (ADT) catalyzes the final step in Phe biosynthesis. This study identified eleven ADT genes in G. hirsutum, twelve in G. barbadense, six in G. arboreum, and six in G. raimondii. Among them, GhADT5 exhibited the highest upregulation under alkali stress. Silencing GhADT5 using virus-induced gene silencing (VIGS) reduced cotton tolerance to alkali stress. GhADT5 silencing also led to decreased plant phenylalanine content, total flavonoid content, and activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD). These reductions caused intracellular accumulation of Malondialdehyde (MDA) and reactive oxygen species (ROS). This oxidative damage ultimately reduced tolerance to alkali stress. In addition, silenced plants displayed reduced stomatal aperture, cellular deformation, and irregular intercellular breaks in the leaf epidermis. In summary, these findings suggest that GhADT5 may enhance resistance to alkali stress by regulating enzymatic and non-enzymatic antioxidant systems. This study highlights the role of GhADT5 under alkali stress and provides novel insights for breeding cotton varieties with improved stress tolerance.

Indexed as

AlkaliesAntioxidantsFlavonoidsGossypiumHydro-LyasesPhenylalaninePlant ProteinsGene Expression Regulation, PlantGene SilencingReactive Oxygen SpeciesStress, PhysiologicalAlkaliesAntioxidantsFlavonoidsHydro-LyasesPhenylalaninePlant ProteinsReactive Oxygen SpeciesArogenate dehydratase (ADT)Cotton breedingCotton (Gossypium Spp.)Flavonoid biosynthesisGhADT5Phenylalanine (phe)Virus-induced gene silencing (VIGS)

Identifiers

PMID39972250
PMCPMC11837375

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