Evidence map›Paper›PMID 39968674›Full record

ArticleAmerican journal of reproductive immunology (New York, N.Y. : 1989)2025

Aging Is Associated With Decreased Lactobacillus and Increased Cervicovaginal Inflammation in Canadian Women.

Ha T Dang, Laura Noel-Romas, Samantha Knodel, Kenzie Birse, Alana Lamont, Kateryna Kratzer, Peter McQueen, Michelle Perner, Hossaena Ayele, Alicia R Berard and 9 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in American journal of reproductive immunology (New York, N.Y. : 1989), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Trial
  2. Review
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Ha T DangCenter for Global Health and Diseases, Department of Pathology, Case Western Reserve University, Cleveland, Ohio, USA.
Laura Noel-RomasCenter for Global Health and Diseases, Department of Pathology, Case Western Reserve University, Cleveland, Ohio, USA.
Samantha KnodelCenter for Global Health and Diseases, Department of Pathology, Case Western Reserve University, Cleveland, Ohio, USA.
Kenzie BirseCenter for Global Health and Diseases, Department of Pathology, Case Western Reserve University, Cleveland, Ohio, USA.
Alana LamontDepartment of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, Manitoba, Canada.
Kateryna KratzerDepartment of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, Manitoba, Canada.
Peter McQueenNational Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
Michelle PernerNational Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
Hossaena AyeleCenter for Global Health and Diseases, Department of Pathology, Case Western Reserve University, Cleveland, Ohio, USA.
Alicia R BerardCenter for Global Health and Diseases, Department of Pathology, Case Western Reserve University, Cleveland, Ohio, USA.
John J SchellenbergDepartment of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, Manitoba, Canada.
Stuart McCorristerNational Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
Garrett WestmacottNational Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
Bonnie SandbergHealth Sciences Centre, Winnipeg, Manitoba, Canada.
Adelicia YuDepartment of Obstetrics, Gynecology & Reproductive Sciences, University of Manitoba, Winnipeg, Manitoba, Canada.
Margaret BurnettDepartment of Obstetrics, Gynecology & Reproductive Sciences, University of Manitoba, Winnipeg, Manitoba, Canada.
Vanessa PoliquinDepartment of Obstetrics, Gynecology & Reproductive Sciences, University of Manitoba, Winnipeg, Manitoba, Canada.
Adam D BurgenerCenter for Global Health and Diseases, Department of Pathology, Case Western Reserve University, Cleveland, Ohio, USA.
Christina Farr ZuendCenter for Global Health and Diseases, Department of Pathology, Case Western Reserve University, Cleveland, Ohio, USA.ORCID 0009-0001-4165-6909

Funding

Case Western Reserve UniversityCIHRInnovative Biomedical and Clinical HIV/AIDS Research HB3-164066
6 · The paper itself

Abstract

problemAging is characterized by a general dysregulation of systemic immune responses that increases susceptibility to infections and malignancies. Immune cells in the female genital tract (FGT) are regulated by sex hormones, but little is known about the impact of aging and menopause on immunology in the FGT. METHOD OF STUDY: This study conducted an age-focused sub-analysis of cervicovaginal samples collected from 47 women enrolled in the Vaginal Mucosal Systems study in Winnipeg, Canada. Paired cervicovaginal lavage and cervical cytobrush were collected and analyzed by Luminex cytokine array, mass spectrometry based metaproteomics, metabolomics, and high dimensional flow cytometry.

resultsThe median age of study participants was 38 (range 19-88), with 12 over the age of 50. Increasing age was significantly correlated with increased cervicovaginal inflammation, including inflammatory cytokine MIP-1β (r = 0.335, p = 0.023), and activated T cells (CD4+HLA-DR+ r = 0406, p = 0.009; CD8+HLA-DR+ r = 0.399, p = 0.010; CD8+CD38+HLA-DR+ r = 0.386, p = 0.013). Proteomic analysis of cervicovaginal mucus identified 925 human proteins, with 108 (11.7%) significantly correlated with age. Pathway analysis indicated biofunctions related to immune response, migration, and myeloid cell phagocytosis increased with age. Interestingly, neutrophil related pathways decreased with age, including G-CSF (r = -0.396, p = 0.006) and reactive oxygen species (z-score = -2.607, p = 2.31E-4). Vaginal Lactobacillus crispatus, a species associated with mucosal health, significantly decreased with age (r = -0.340, p = 0.022), with participants over the age of 50 more likely to have non-Lactobacillus dominant microbiomes compared to those under 40.

conclusionsTogether, our data suggests that there is an increase in cervicovaginal inflammation and a decrease in L. crispatus that occurs with aging.

Indexed as

AgingCervix UteriInflammationLactobacillusVaginaAdultAgedAged, 80 and overCanadaCytokinesFemaleHumansMiddle AgedYoung AdultCytokinesagingcervicovaginal microenvironmentwomen's health

Identifiers

PMID39968674
PMCPMC11836769

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.