Evidence map›Paper›PMID 39967781›Full record

ArticleRegenerative biomaterials2025

Material surface conjugated with fibroblast growth factor-2 for pluripotent stem cell culture and differentiation.

Tzu-Cheng Sung, Zhi-Xian Pan, Ting Wang, Hui-Yu Lin, Chia-Lun Chang, Ling-Chun Hung, Suresh Kumar Subbiah, Remya Rajan Renuka, Shih-Jie Chou, Shih-Hwa Chiou and 4 more

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In one paragraph

Article in Regenerative biomaterials, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

14 authors.

Tzu-Cheng SungState Key Laboratory of Ophthalmology, Optometry and Visual Science, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
Zhi-Xian PanDepartment of Chemical and Materials Engineering, National Central University, Taoyuan 32001, Taiwan, ROC.
Ting WangState Key Laboratory of Ophthalmology, Optometry and Visual Science, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.ORCID https://orcid.org/0000-0002-0961-8224
Hui-Yu LinDepartment of Chemical and Materials Engineering, National Central University, Taoyuan 32001, Taiwan, ROC.
Chia-Lun ChangDepartment of Chemical and Materials Engineering, National Central University, Taoyuan 32001, Taiwan, ROC.
Ling-Chun HungDepartment of Chemical and Materials Engineering, National Central University, Taoyuan 32001, Taiwan, ROC.
Suresh Kumar SubbiahCentre for Stem Cell Mediated Advanced Research Therapeutics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai 600 077, Tamil Nadu, India.
Remya Rajan RenukaCentre for Stem Cell Mediated Advanced Research Therapeutics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai 600 077, Tamil Nadu, India.
Shih-Jie ChouDepartment of Medical Research, Taipei Veterans General Hospital, Taipei 112201, Taiwan, ROC.
Shih-Hwa ChiouDepartment of Medical Research, Taipei Veterans General Hospital, Taipei 112201, Taiwan, ROC.ORCID https://orcid.org/0000-0002-2481-5357
Idaszek JoannaDivision of Materials Design, Faculty of Materials Science and Engineering, Warsaw University of Technology, Warsaw 02-507, Poland.
Henry Hsin-Chung LeeDepartment of Surgery, Hsinchu Cathay General Hospital, Hsinchu 30060, Taiwan, ROC.
Gwo-Jang WuGraduate Institute of Medical Sciences and Department of Obstetrics & Gynecology, Tri-Service General Hospital, National Defense Medical Center, Taipei 11490, Taiwan, ROC.
Akon HiguchiState Key Laboratory of Ophthalmology, Optometry and Visual Science, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.ORCID https://orcid.org/0000-0003-2970-8531

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fibroblast growth factor-2 (FGF-2) is a critical molecule for sustaining the pluripotency of human pluripotent stem (PS) cells. However, FGF-2 is extremely unstable and cannot be stored long periods at room temperature. Therefore, the following FGF-2-conjugated cell culture materials were developed to stabilize FGF-2: FGF-2-conjugated polyvinyl alcohol (PVAI-C-FGF) hydrogels and FGF-2-conjugated carboxymethyl cellulose-coated (CMC-C-FGF) dishes. Human induced pluripotent stem (iPS) cells were proliferated on recombinant vitronectin (rVN)-coated PVAI-C-FGF hydrogels and CMC-C-FGF dishes in medium without FGF-2. Human iPS cells could not be cultivated on rVN-coated PVAI-C-FGF hydrogels for more than two passages but could proliferate on rVN-coated CMC-C-FGF dishes. These results indicated that the amount of immobilized FGF-2 and the base cell materials are important, including the amount of immobilized rVN and the conformation of FGF-2 on the surfaces. When human iPS cells were proliferated on rVN-coated CMC-C-FGF surfaces in medium containing no FGF-2 for 10 passages, their pluripotency and potential to differentiate into cells originating from three germ layers were maintained

Indexed as

carboxymethyl cellulosecell differentiationfibroblast growth factor-2human pluripotent stem cellsmaterial design

Identifiers

PMID39967781
PMCPMC11835233

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.