Evidence map›Paper›PMID 39966555›Full record

ArticleScientific reports2025

High-sensitivity qPCR detection method based on silver flower-like LSPR-active material.

Congxi Song, Keliang Li, Zhihui Jiang, Yibo Tang, Wusheng Hu, Hongmin Mao, Xin Zhao, Jian Yin, Huanjun Lu, Zhaoliang Cao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Congxi Song *Key Laboratory of Intelligent Optoelectronic Devices and Chips of Jiangsu Higher Education Institutions, School of Physical Science and Technology, Suzhou University of Science and Technology, Suzhou, 215009, China.
Keliang Li *Key Laboratory of Intelligent Optoelectronic Devices and Chips of Jiangsu Higher Education Institutions, School of Physical Science and Technology, Suzhou University of Science and Technology, Suzhou, 215009, China.
Zhihui JiangKey Laboratory of Intelligent Optoelectronic Devices and Chips of Jiangsu Higher Education Institutions, School of Physical Science and Technology, Suzhou University of Science and Technology, Suzhou, 215009, China.
Yibo TangKey Laboratory of Intelligent Optoelectronic Devices and Chips of Jiangsu Higher Education Institutions, School of Physical Science and Technology, Suzhou University of Science and Technology, Suzhou, 215009, China.
Wusheng HuBasic Department, Jiyuan Vocational and Technical College, Jiyuan, 454682, China.
Hongmin MaoKey Laboratory of Intelligent Optoelectronic Devices and Chips of Jiangsu Higher Education Institutions, School of Physical Science and Technology, Suzhou University of Science and Technology, Suzhou, 215009, China.
Xin ZhaoSchool of Chemistry and Life Sciences, Suzhou University of Science and Technology, Suzhou, 215009, China.
Jian YinCAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, 215163, Jiangsu, People's Republic of China. yinj@sibet.ac.cn.
Huanjun LuKey Laboratory of Intelligent Optoelectronic Devices and Chips of Jiangsu Higher Education Institutions, School of Physical Science and Technology, Suzhou University of Science and Technology, Suzhou, 215009, China. luhuanjun@usts.edu.cn.
Zhaoliang CaoKey Laboratory of Intelligent Optoelectronic Devices and Chips of Jiangsu Higher Education Institutions, School of Physical Science and Technology, Suzhou University of Science and Technology, Suzhou, 215009, China. caozl@usts.edu.cn.

Funding

the National Natural Science Foundation of China Grants No. 22205155
6 · The paper itself

Abstract

Quantitative polymerase chain reaction (qPCR) technology offers advantages such as easy operation and high specificity, but also faces issues like lengthy detection time and low sensitivity. This paper proposes a method by using novel SERS-active silver flower-like material to enhance the fluorescence signal generated by qPCR instrument, so as to shorten the detection time and improve the detection sensitivity. In this method, silver flower-like material is first prepared on substrates using a lyotropic liquid crystal soft template combined with electrodeposition growth. Then, an ultrasonic device is used to peel the active material into aqueous solution, and the peeling efficiency reaches 90%. Using rhodamine 6G (R6G) as the qPCR amplification detection molecule, the fluorescence signal generated from the flower-like material-containing sample is increased by 20%. Finally, the qPCR amplification effect is detected with the commercially available new coronavirus nucleic acid detection kit. The results showed that the CT value decreased from 35 to 31 after adding the silver flower-like material, and the detection efficiency increased by 20%. Meanwhile, no non-specific amplification is observed. For COVID-19 samples at S7 concentration, the positive detection rate rises from 1/200 to 2/3 by our method.

Indexed as

COVID-19Real-Time Polymerase Chain ReactionSARS-CoV-2SilverSpectrum Analysis, RamanHumansLimit of DetectionRhodaminesSensitivity and Specificityrhodamine 6GRhodaminesSilver

Identifiers

PMID39966555
PMCPMC11836446

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.